Molecular Endocrinology, Vol 6, 2219-2228, Copyright © 1992 by Endocrine Society

ARTICLES

Insulin rapidly decreases insulin-like growth factor-binding protein-1 gene transcription in streptozotocin-diabetic rats

GT Ooi, LY Tseng, MQ Tran and MM Rechler
Growth and Development Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.

Insulin-like growth factor-binding protein-1 (IGFBP-1) can inhibit or potentiate IGF action. The biological activity of IGFBP-1 is determined by many factors, including its abundance in tissues and plasma, posttranslational modifications, and localization. IGFBP-1 levels in human plasma are highly regulated. They are increased after acute fasting and in diabetes, and are rapidly reversed by refeeding and insulin treatment, respectively. Similarly, IGFBP-1 mRNA is increased in the liver of severely diabetic and ketotic rats and decreased after 4 days of insulin treatment. Insulin rapidly decreases IGFBP-1 mRNA and IGFBP-1 transcription in rat hepatoma cells. The present study asks whether the increase in IGFBP-1 mRNA in diabetic rat liver reflects increased gene transcription, whether insulin decreases IGFBP-1 mRNA through a transcriptional or posttranscriptional mechanism, and whether this decrease is sufficiently rapid to account for the dynamic fluctuations in plasma IGFBP-1. Rats were injected ip with 100 mg/kg streptozotocin and used 7 days later when they were hyperglycemic and failed to gain weight, but were not ketotic. Hepatic IGFBP-1 mRNA levels were 13.6 +/- 5.3-fold greater in diabetic than control liver and decreased to the low levels in nondiabetic controls within 1 h after insulin treatment. In run-on transcription assays, IGFBP-1 transcription was 12.6 +/- 1.5-fold greater in nuclei from diabetic than control liver and decreased to low control levels by 1 h after insulin injection. Normalization of hepatic IGFBP-1 mRNA in insulin- treated diabetic animals did not require restoration of euglycemia. IGFBP-1 mRNA and IGFBP-1 gene transcription also were increased in the kidney of diabetic ketotic rats. We propose that the dynamic regulation of IGFBP-1 gene transcription in diabetes and after insulin treatment, by determining the availability of IGFBP-1 in tissues and plasma, may be a critical factor in the modulation of IGF action.

This article has been cited by other articles:

				S. C. Martinez, K. Tanabe, C. Cras-Meneur, N. A. Abumrad, E. Bernal-Mizrachi, and M. A. Permutt Inhibition of Foxo1 Protects Pancreatic Islet {beta}-Cells Against Fatty Acid and Endoplasmic Reticulum Stress-Induced Apoptosis Diabetes, April 1, 2008; 57(4): 846 - 859. [Abstract] [Full Text] [PDF]

				S. C. Martinez, C. Cras-Meneur, E. Bernal-Mizrachi, and M. A. Permutt Glucose Regulates Foxo1 Through Insulin Receptor Signaling in the Pancreatic Islet {beta}-cell Diabetes, June 1, 2006; 55(6): 1581 - 1591. [Abstract] [Full Text] [PDF]

				E. K. Wei, J. Ma, M. N. Pollak, N. Rifai, C. S. Fuchs, S. E. Hankinson, and E. Giovannucci C-Peptide, Insulin-like Growth Factor Binding Protein-1, Glycosylated Hemoglobin, and the Risk of Distal Colorectal Adenoma in Women. Cancer Epidemiol. Biomarkers Prev., April 1, 2006; 15(4): 750 - 755. [Abstract] [Full Text] [PDF]

				P. Froment, C. Staub, S. Hembert, C. Pisselet, M. Magistrini, B. Delaleu, D. Seurin, J. E. Levine, L. Johnson, M. Binoux, et al. Reproductive Abnormalities in Human Insulin-Like Growth Factor-Binding Protein-1 Transgenic Male Mice Endocrinology, April 1, 2004; 145(4): 2080 - 2091. [Abstract] [Full Text] [PDF]

				E. Giovannucci Obesity, insulin, and colon cancer. AACR Meeting Abstracts, March 1, 2004; 2004(1): 1319 - 1320. [Abstract]

				W.-C. Tsai, N. Bhattacharyya, L.-Y. Han, J. A. Hanover, and M. M. Rechler Insulin Inhibition of Transcription Stimulated by the Forkhead Protein Foxo1 Is Not Solely due to Nuclear Exclusion Endocrinology, December 1, 2003; 144(12): 5615 - 5622. [Abstract] [Full Text] [PDF]

				S.-E. Kong, S. M. Firth, R. C. Baxter, and P. J. D. Delhanty Regulation of the acid-labile subunit in sustained endotoxemia Am J Physiol Endocrinol Metab, October 1, 2002; 283(4): E692 - E701. [Abstract] [Full Text] [PDF]

				T. J. Maures and C. Duan Structure, Developmental Expression, and Physiological Regulation of Zebrafish IGF Binding Protein-1 Endocrinology, July 1, 2002; 143(7): 2722 - 2731. [Abstract] [Full Text] [PDF]

				L. E. L. Katz, D. D. DeLeon, H. Zhao, and A. F. Jawad Free and Total Insulin-Like Growth Factor (IGF)-I Levels Decline during Fasting: Relationships with Insulin and IGF-Binding Protein-1 J. Clin. Endocrinol. Metab., June 1, 2002; 87(6): 2978 - 2983. [Abstract] [Full Text] [PDF]

				P. Froment, D. Seurin, S. Hembert, J. E. Levine, C. Pisselet, D. Monniaux, M. Binoux, and P. Monget Reproductive Abnormalities in Human IGF Binding Protein-1 Transgenic Female Mice Endocrinology, May 1, 2002; 143(5): 1801 - 1808. [Abstract] [Full Text] [PDF]

				T. Matsukawa, Y. Inoue, Y. Oishi, H. Kato, and T. Noguchi Up-Regulation of Upstream Stimulatory Factors by Protein Malnutrition and Its Possible Role in Regulation of the IGF-Binding Protein-1 Gene Endocrinology, November 1, 2001; 142(11): 4643 - 4651. [Abstract] [Full Text] [PDF]

				E. Giovannucci Insulin, Insulin-Like Growth Factors and Colon Cancer: A Review of the Evidence J. Nutr., November 1, 2001; 131(11): 3109S - 3120. [Abstract] [Full Text] [PDF]

				S. Yakar, J.-L. Liu, A. M. Fernandez, Y. Wu, A. V. Schally, J. Frystyk, S. D. Chernausek, W. Mejia, and D. Le Roith Liver-Specific igf-1 Gene Deletion Leads to Muscle Insulin Insensitivity Diabetes, May 1, 2001; 50(5): 1110 - 1118. [Abstract] [Full Text]

				E. Giovannucci, M. N. Pollak, E. A. Platz, W. C. Willett, M. J. Stampfer, N. Majeed, G. A. Colditz, F. E. Speizer, and S. E. Hankinson A Prospective Study of Plasma Insulin-like Growth Factor-1 and Binding Protein-3 and Risk of Colorectal Neoplasia in Women Cancer Epidemiol. Biomarkers Prev., April 1, 2000; 9(4): 345 - 349. [Abstract] [Full Text]

				L. A. Bach, R. Dean, S. Youssef, and M. E. Cooper Aminoguanidine ameliorates changes in the IGF system in experimental diabetic nephropathy Nephrol. Dial. Transplant., March 1, 2000; 15(3): 347 - 354. [Abstract] [Full Text] [PDF]

				J. Frystyk, P. J. D. Delhanty, C. Skjarbak, and R. C. Baxter Changes in the circulating IGF system during short-term fasting and refeeding in rats Am J Physiol Endocrinol Metab, August 1, 1999; 277(2): E245 - E252. [Abstract] [Full Text] [PDF]

				G. Schweizer-Groyer, N. Jibard, E. Neau, D. Fortin, F. Cadepond, E.-E. Baulieu, and A. Groyer The Glucocorticoid Response Element II Is Functionally Homologous in Rat and Human Insulin-like Growth Factor-binding Protein-1 Promoters J. Biol. Chem., April 23, 1999; 274(17): 11679 - 11686. [Abstract] [Full Text] [PDF]

				T. A. Jacot and D. R. Clemmons Effect of Glucose on Insulin-Like Growth Factor Binding Protein-4 Proteolysis Endocrinology, January 1, 1998; 139(1): 44 - 50. [Abstract] [Full Text] [PDF]