| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Molecular Endocrinology, Vol 6, 320-326, Copyright © 1992 by Endocrine Society
ARTICLES |
H Wang, S Nelson, M Ascoli and DL Segaloff
Department of Physiology, University of Iowa College of Medicine, Iowa City 52242.
The LH/CG receptor is a G protein-coupled receptor present on gonadal cells whose levels are modulated by a number of hormones, growth factors, and second messenger analogs. With the recently cloned cDNA for the LH/CG receptor, it has been shown that changes in the levels of the cognate mRNA are involved, at least in part, in the observed changes in receptor density. In order to study the transcriptional regulation of the LH/CG receptor we have isolated a 2-kilobase region of the 5'-flanking region of the rat LH/CG receptor gene and subcloned nucleotide -1 (relative to the translational initiation codon) to -1370 into a luciferase reporter plasmid. We show here that this region of the LH/CG receptor gene is able to enhance luciferase activity in MA-10 cells, a line of Leydig tumor cells that normally express LH/CG receptors, as opposed to human kidney 293 cells, which do not. Furthermore, the addition of 8-bromo-cAMP to MA-10 cells, under conditions known to decrease LH/CG receptor numbers and receptor mRNA levels, decreases the relative luciferase activity to about 26% of control. This decrease in reporter gene activity is severely blunted in a subclone of MA-10 cells with a cAMP-resistant phenotype. Our studies show, for the first time, that sequence(s) present with 1370 base pairs of the translational start site of the rat LH/CG receptor gene are sufficient for conferring expression of this gene in Leydig cells and for the negative modulation of LH/CG receptor gene transcription by high concentrations of cAMP.
This article has been cited by other articles:
![]() |
S. Ikeda, K. Nakamura, K. Kogure, Y. Omori, S. Yamashita, K. Kubota, T. Mizutani, K. Miyamoto, and T. Minegishi Effect of Estrogen on the Expression of Luteinizing Hormone-Human Chorionic Gonadotropin Receptor Messenger Ribonucleic Acid in Cultured Rat Granulosa Cells Endocrinology, April 1, 2008; 149(4): 1524 - 1533. [Abstract] [Full Text] [PDF] |
||||
![]() |
R S Bhaskaran and M Ascoli The post-endocytotic fate of the gonadotropin receptors is an important determinant of the desensitization of gonadotropin responses J. Mol. Endocrinol., April 1, 2005; 34(2): 447 - 457. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Hamalainen, J. Kero, M. Poutanen, and I. Huhtaniemi Transgenic Mice Harboring Murine Luteinizing Hormone Receptor Promoter/{beta}-Galactosidase Fusion Genes: Different Structural and Hormonal Requirements of Expression in the Testis, Ovary, and Adrenal Gland Endocrinology, October 1, 2002; 143(10): 4096 - 4103. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Inoue, K. Nakamura, K. Abe, T. Hirakawa, M. Tsuchiya, H. Matsuda, K. Miyamoto, and T. Minegishi Effect of Transforming Growth Factor {beta} on the Expression of Luteinizing Hormone Receptor in Cultured Rat Granulosa Cells Biol Reprod, August 1, 2002; 67(2): 610 - 615. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Yoshino, T. Mizutani, K. Yamada, M. Tsuchiya, T. Minegishi, T. Yazawa, H. Kawata, T. Sekiguchi, T. Kajitani, and K. Miyamoto Early Growth Response Gene-1 Regulates the Expression of the Rat Luteinizing Hormone Receptor Gene Biol Reprod, June 1, 2002; 66(6): 1813 - 1819. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Ascoli, F. Fanelli, and D. L. Segaloff The Lutropin/Choriogonadotropin Receptor, A 2002 Perspective Endocr. Rev., April 1, 2002; 23(2): 141 - 174. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Hamalainen, M. Poutanen, and I. Huhtaniemi Promoter Function of Different Lengths of the Murine Luteinizing Hormone Receptor Gene 5'-Flanking Region in Transfected Gonadal Cells and in Transgenic Mice Endocrinology, June 1, 2001; 142(6): 2427 - 2434. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Chen, X. Liu, and D. L. Segaloff Identification of an SAS (Sp1c Adjacent Site)-Like Element in the Distal 5'-Flanking Region of the Rat Lutropin Receptor Gene Essential for Cyclic Adenosine 3',5'-Monophosphate Responsiveness Endocrinology, May 1, 2001; 142(5): 2013 - 2021. [Abstract] [Full Text] |
||||
![]() |
S. Chen, X. Liu, and D. L. Segaloff A Novel Cyclic Adenosine 3',5'-Monophosphate-Responsive Element Involved In the Transcriptional Regulation of the Lutropin Receptor Gene in Granulosa Cells Mol. Endocrinol., September 1, 2000; 14(9): 1498 - 1508. [Abstract] [Full Text] |
||||
![]() |
T. Hirakawa, T. Minegishi, K. Abe, H. Kishi, Y. Ibuki, and K. Miyamoto A Role of Insulin-Like Growth Factor I in Luteinizing Hormone Receptor Expression in Granulosa Cells Endocrinology, November 1, 1999; 140(11): 4965 - 4971. [Abstract] [Full Text] |
||||
![]() |
T. Hämäläinen, M. Poutanen, and I. Huhtaniemi Age- and Sex-Specific Promoter Function of a 2-Kilobase 5'-Flanking Sequence of the Murine Luteinizing Hormone Receptor Gene in Transgenic Mice Endocrinology, November 1, 1999; 140(11): 5322 - 5329. [Abstract] [Full Text] |
||||
![]() |
T. Hirakawa, T. Minegishi, M. Tano, T. Kameda, H. Kishi, Y. Ibuki, T. Mizutani, and K. Miyamoto Effect of Prolactin on the Expression of Luteinizing Hormone Receptors during Cell Differentiation in Cultured Rat Granulosa Cells Endocrinology, August 1, 1999; 140(8): 3444 - 3451. [Abstract] [Full Text] |
||||
![]() |
S. Chen, H. Shi, X. Liu, and D. L. Segaloff Multiple Elements and Protein Factors Coordinate the Basal and Cyclic Adenosine 3',5'-Monophosphate-Induced Transcription of the Lutropin Receptor Gene in Rat Granulosa Cells Endocrinology, May 1, 1999; 140(5): 2100 - 2109. [Abstract] [Full Text] |
||||
![]() |
H. Kaji, S. Tai, Y. Okimura, G. Iguchi, Y. Takahashi, H. Abe, and K. Chihara Cloning and Characterization of the 5'-Flanking Region of the Human Growth Hormone Secretagogue Receptor Gene J. Biol. Chem., December 18, 1998; 273(51): 33885 - 33888. [Abstract] [Full Text] [PDF] |
||||
![]() |
F.-P. Zhang, T. El-Hafnawy, and I. Huhtaniemi Regulation of Luteinizing Hormone Receptor Gene Expression by Insulin-Like Growth Factor-I in an Immortalized Murine Leydig Tumor Cell Line (BLT-1) Biol Reprod, November 1, 1998; 59(5): 1116 - 1123. [Abstract] [Full Text] |
||||
![]() |
T. Iwasaki, M. Yamada, T. Satoh, S. Konaka, Y. Ren, K. Hashimoto, H. Kohga, Y. Kato, and M. Mori Genomic Organization and Promoter Function of the Human Thyrotropin-releasing Hormone Receptor Gene J. Biol. Chem., September 6, 1996; 271(36): 22183 - 22188. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |