Molecular Endocrinology, Vol 6, 1135-1141, Copyright © 1992 by Endocrine Society

ARTICLES

CCAAT/enhancer binding protein expression is rapidly extinguished in TA1 adipocyte cells treated with tumor necrosis factor

PM Williams, DJ Chang, U Danesch, GM Ringold and RA Heller
Institute of Cancer and Developmental Biology, Syntex Research, Palo Alto, California 94303.

Tumor necrosis factor (TNF) has been shown to have diverse effects on a wide variety of cell types. In the mouse adipogenic TA1 cell line, TNF completely abolishes differentiation and reverts fully differentiated fat cells into fibroblasts. This block in differentiation and its reversal is due to the rapid reduction in the expression of adipose- specific genes. This study reports that the transcription factor, CCAAT/enhancer binding protein (C/EBP), previously reported to promote the differentiation of 3T3-L1 adipocytes, is expressed in TA1 cells. During their growth in culture, the levels of C/EBP, as evidenced by its cellular levels of specific mRNA, protein, and DNA binding activity, increase dramatically when cells reach confluence and proceed to differentiate. Addition of TNF to cultured preadipocytes or fully differentiated adipocytes rapidly reduces C/EBP levels and is accompanied by the decrease in expression of adipose-specific genes. C/EBP binding sites occur in several adipose-specific genes, and here it is demonstrated that its presence in a novel adipose-specific gene, Clone 47, also referred to as FSP27, may be responsible for the strong down-regulation of the expression of the Clone 47 (FSP27) promoter- linked chloramphenicol acetyl transferase gene by TNF. This study proposes that the loss of C/EBP in response to TNF treatment may in part explain the loss of the adipocyte differentiated state.

This article has been cited by other articles:

				J. Y. Kim, K. Liu, S. Zhou, K. Tillison, Y. Wu, and C. M. Smas Assessment of fat-specific protein 27 in the adipocyte lineage suggests a dual role for FSP27 in adipocyte metabolism and cell death Am J Physiol Endocrinol Metab, April 1, 2008; 294(4): E654 - E667. [Abstract] [Full Text] [PDF]

				V. Puri, S. Konda, S. Ranjit, M. Aouadi, A. Chawla, M. Chouinard, A. Chakladar, and M. P. Czech Fat-specific Protein 27, a Novel Lipid Droplet Protein That Enhances Triglyceride Storage J. Biol. Chem., November 23, 2007; 282(47): 34213 - 34218. [Abstract] [Full Text] [PDF]

				E. A. Nordstrom, M. Ryden, E. C. Backlund, I. Dahlman, M. Kaaman, L. Blomqvist, B. Cannon, J. Nedergaard, and P. Arner A Human-Specific Role of Cell Death-Inducing DFFA (DNA Fragmentation Factor-{alpha})-Like Effector A (CIDEA) in Adipocyte Lipolysis and Obesity Diabetes, June 1, 2005; 54(6): 1726 - 1734. [Abstract] [Full Text] [PDF]

				J. M. Brown, M. S. Boysen, S. Chung, O. Fabiyi, R. F. Morrison, S. Mandrup, and M. K. McIntosh Conjugated Linoleic Acid Induces Human Adipocyte Delipidation: AUTOCRINE/PARACRINE REGULATION OF MEK/ERK SIGNALING BY ADIPOCYTOKINES J. Biol. Chem., June 18, 2004; 279(25): 26735 - 26747. [Abstract] [Full Text] [PDF]

				F. M. GREGOIRE, C. M. SMAS, and H. S. SUL Understanding Adipocyte Differentiation Physiol Rev, July 1, 1998; 78(3): 783 - 809. [Abstract] [Full Text] [PDF]

				A. M. Valverde, T. Teruel, P. Navarro, M. Benito, and M. Lorenzo Tumor Necrosis Factor-{alpha} Causes Insulin Receptor Substrate-2-Mediated Insulin Resistance and Inhibits Insulin-Induced Adipogenesis in Fetal Brown Adipocytes Endocrinology, March 1, 1998; 139(3): 1229 - 1238. [Abstract] [Full Text] [PDF]

				K. E. Hadri, A. Courtalon, X. Gauthereau, A.-M. Chambaut-Guerin, J. Pairault, and B. Feve Differential Regulation by Tumor Necrosis Factor-alpha of beta 1-, beta 2-, and beta 3-Adrenoreceptor Gene Expression in 3T3-F442A Adipocytes J. Biol. Chem., September 26, 1997; 272(39): 24514 - 24521. [Abstract] [Full Text] [PDF]

				H. Xing, J. P. Northrop, J. R. Grove, K. E. Kilpatrick, J.-L. Su, and G. M. Ringold TNF{alpha}-Mediated Inhibition and Reversal of Adipocyte Differentiation Is Accompanied by Suppressed Expression of PPAR{gamma} without Effects on Pref-1 Expression Endocrinology, July 1, 1997; 138(7): 2776 - 2783. [Abstract] [Full Text] [PDF]