Molecular Endocrinology, Vol 6, 1163-1169, Copyright © 1992 by Endocrine Society

ARTICLES

Cloning and functional expression of a mouse gonadotropin-releasing hormone receptor

M Tsutsumi, W Zhou, RP Millar, PL Mellon, JL Roberts, CA Flanagan, K Dong, B Gillo and SC Sealfon
Fishberg Research Center in Neurobiology, Mount Sinai School of Medicine, New York, New York 10029.

GnRH plays a pivotal role in the reproductive system, and GnRH analogs have wide therapeutic applications ranging from the treatment of prostatic cancer to infertility. Determination of the predicted structure of the GnRH receptor (GnRHR) would illuminate the mechanisms of receptor activation and regulation and allow directed design of improved GnRH analogs. We report the cloning of a cDNA representing the mouse GnRHR and confirm its identity using Xenopus oocyte expression. Injection of sense RNA transcript leads to the expression of a functional, high affinity GnRHR. Expression of the GnRHR using gonadotrope cell line RNA, however, is blocked by an antisense oligonucleotide. In situ hybridization in the rat anterior pituitary reveals a characteristic GnRHR distribution. The nucleotide sequence encodes a 327-amino acid protein which has the seven putative transmembrane domains characteristic of G protein-coupled receptors, but which lacks a typical intracellular C-terminus. The unusual structure and novel potential regulatory domain of the GnRHR may explain unique aspects of its signal transduction and regulation.

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				K. Rao, W.-Y. Paik, L. Zheng, R. M. Jobin, M. Tomic, H. Jiang, S. Nakanishi, and S. S. Stojilkovic Wortmannin-Sensitive and -Insensitive Steps in Calcium-Controlled Exocytosis in Pituitary Gonadotrophs: Evidence That Myosin Light Chain Kinase Mediates Calcium-Dependent and Wortmannin-Sensitive Gonadotropin Secretion Endocrinology, April 1, 1997; 138(4): 1440 - 1449. [Abstract] [Full Text] [PDF]

				U. B. Kaiser, P. M. Conn, and W. W. Chin Studies of Gonadotropin-Releasing Hormone (GnRH) Action Using GnRH Receptor-Expressing Pituitary Cell Lines Endocr. Rev., February 1, 1997; 18(1): 46 - 70. [Abstract] [Full Text]

				C. A. McArdle, G. B. Willars, R. C. Fowkes, S. R. Nahorski, J. S. Davidson, and W. Forrest-Owen Desensitization of Gonadotropin-releasing Hormone Action in alpha T3-1 Cells Due to Uncoupling of Inositol 1,4,5-Trisphosphate Generation and Ca2+ Mobilization J. Biol. Chem., September 27, 1996; 271(39): 23711 - 23717. [Abstract] [Full Text] [PDF]

				J. S. Davidson, C. A. McArdle, P. Davies, R. Elario, C. A. Flanagan, and R. P. Millar Asn102 of the Gonadotropin-releasing Hormone Receptor Is a Critical Determinant of Potency for Agonists Containing C-terminal Glycinamide J. Biol. Chem., June 28, 1996; 271(26): 15510 - 15514. [Abstract] [Full Text] [PDF]

				E. Alarid, J. Windle, D. Whyte, and P. Mellon Immortalization of pituitary cells at discrete stages of development by directed oncogenesis in transgenic mice Development, January 10, 1996; 122(10): 3319 - 3329. [Abstract] [PDF]

				K. K. Arora, A. Sakai, and K. J. Catt Effects of Second Intracellular Loop Mutations on Signal Transduction and Internalization of the Gonadotropin-releasing Hormone Receptor J. Biol. Chem., September 29, 1995; 270(39): 22820 - 22826. [Abstract] [Full Text] [PDF]

				W. Zhou, V. Rodic, S. Kitanovic, C. A. Flanagan, L. Chi, H. Weinstein, S. Maayani, R. P. Millar, and S. C. Sealfon A Locus of the Gonadotropin-releasing Hormone Receptor That Differentiates Agonist and Antagonist Binding Sites J. Biol. Chem., August 11, 1995; 270(32): 18853 - 18857. [Abstract] [Full Text] [PDF]

				H A Ingraham, D S Lala, Y Ikeda, X Luo, W H Shen, M W Nachtigal, R Abbud, J H Nilson, and K L Parker The nuclear receptor steroidogenic factor 1 acts at multiple levels of the reproductive axis. Genes & Dev., October 1, 1994; 8(19): 2302 - 2312. [Abstract] [PDF]

				D Lew, H Brady, K Klausing, K Yaginuma, L E Theill, C Stauber, M Karin, and P L Mellon GHF-1-promoter-targeted immortalization of a somatotropic progenitor cell results in dwarfism in transgenic mice. Genes & Dev., April 1, 1993; 7(4): 683 - 693. [Abstract] [PDF]

				T. Yokoi, M. Ohmichi, K. Tasaka, A. Kimura, Y. Kanda, J. Hayakawa, M. Tahara, K. Hisamoto, H. Kurachi, and Y. Murata Activation of the Luteinizing Hormone beta Promoter by Gonadotropin-releasing Hormone Requires c-Jun NH2-terminal Protein Kinase J. Biol. Chem., July 7, 2000; 275(28): 21639 - 21647. [Abstract] [Full Text] [PDF]

				L. Wang, J. Bogerd, H. S. Choi, J. Y. Seong, J. M. Soh, S. Y. Chun, M. Blomenrohr, B. E. Troskie, R. P. Millar, W. H. Yu, et al. Three distinct types of GnRH receptor characterized in the bullfrog PNAS, January 2, 2001; 98(1): 361 - 366. [Abstract] [Full Text] [PDF]