Molecular Endocrinology, Vol 7, 181-188, Copyright © 1993 by Endocrine Society

ARTICLES

Trophoblast-specific transcription from the mouse placental lactogen-I gene promoter

MM Shida, YK Ng, MJ Soares and DI Linzer
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208.

We have isolated the gene encoding mouse placental lactogen-I and characterized the promoter region of this gene by transient and stable transfection. Promoter sequences extending 274 basepairs (bp) up-stream from the start site of transcription contain all of the elements necessary for maximal expression upon transient transfection into the rat choriocarcinoma Rcho-1 cell line; these Rcho-1 cultures contain both proliferative trophoblast stem cells and terminally differentiated trophoblast giant cells. In stably transfected cell lines, expression from this promoter increases as the percentage of differentiated cells in the culture increases. In contrast to these results in trophoblast cells, the 274-bp promoter as well as a promoter region extending 2700 bp up-stream of the transcriptional start site are unable to drive transcription in a variety of other cell types. Mutational and protein binding analyses indicate that two AP-1 sites are required for maximal expression in Rcho-1 cells, and that the composition of the AP-1 transcription factor may vary as differentiation in the cell culture increases. In addition to these two AP-1 sites, at least one other element appears to be critical for promoter activity in trophoblast cells.

This article has been cited by other articles:

				K. Asanoma, H. Kato, S. Yamaguchi, C. H. Shin, Z.-P. Liu, K. Kato, T. Inoue, Y. Miyanari, K. Yoshikawa, K. Sonoda, et al. HOP/NECC1, A Novel Regulator of Mouse Trophoblast Differentiation J. Biol. Chem., August 17, 2007; 282(33): 24065 - 24074. [Abstract] [Full Text] [PDF]

				D. Ghosh, S. Sachdev, M. Hannink, and R. M. Roberts Coordinate Regulation of Basal and Cyclic 5'-Adenosine Monophosphate (cAMP)-Activated Expression of Human Chorionic Gonadotropin-{alpha} by Ets-2 and cAMP-Responsive Element Binding Protein Mol. Endocrinol., April 1, 2005; 19(4): 1049 - 1066. [Abstract] [Full Text] [PDF]

				A. Ozturk, A. Fresnoza, A. Savoie, H. W. Duckworth, and M. L. Duckworth Defining Regulatory Regions in the Rat Prolactin Gene Family Locus Using a Large P1 Genomic Clone Endocrinology, November 1, 2003; 144(11): 4742 - 4754. [Abstract] [Full Text] [PDF]

				D. O. Wiemers, L.-j. Shao, R. Ain, G. Dai, and M. J. Soares The Mouse Prolactin Gene Family Locus Endocrinology, January 1, 2003; 144(1): 313 - 325. [Abstract] [Full Text] [PDF]

				D. Ghosh, T. Ezashi, M. C. Ostrowski, and R. M. Roberts A Central Role for Ets-2 in the Transcriptional Regulation and Cyclic Adenosine 5'-Monophosphate Responsiveness of the Human Chorionic Gonadotropin-{beta} Subunit Gene Mol. Endocrinol., January 1, 2003; 17(1): 11 - 26. [Abstract] [Full Text] [PDF]

				J. C. Cross, L. Anson-Cartwright, and I. C. Scott Transcription Factors Underlying the Development and Endocrine Functions of the Placenta Recent Prog. Horm. Res., January 1, 2002; 57(1): 221 - 234. [Abstract] [Full Text] [PDF]

				J.-H. Cho, H. Kimura, T. Minami, J. Ohgane, N. Hattori, S. Tanaka, and K. Shiota DNA Methylation Regulates Placental Lactogen I Gene Expression Endocrinology, August 1, 2001; 142(8): 3389 - 3396. [Abstract] [Full Text] [PDF]

				K. W. Cheng, B. K. C. Chow, and P. C. K. Leung Functional Mapping of a Placenta-Specific Upstream Promoter for Human Gonadotropin-Releasing Hormone Receptor Gene Endocrinology, April 1, 2001; 142(4): 1506 - 1516. [Abstract] [Full Text]

				G. Dai, M. W. Wolfe, and M. J. Soares Distinct Regulatory Regions from the Prolactin-Like Protein C Variant Promoter Direct Trophoblast Giant Cell Versus Spongiotrophoblast Cell-Specific Expression Endocrinology, October 1, 1999; 140(10): 4691 - 4698. [Abstract] [Full Text]

				K. E. Orwig and M. J. Soares Transcriptional Activation of the Decidual/Trophoblast Prolactin-Related Protein Gene Endocrinology, September 1, 1999; 140(9): 4032 - 4039. [Abstract] [Full Text]

				Y. Lee and J. L. Voogt Feedback Effects of Placental Lactogens on Prolactin Levels and Fos-Related Antigen Immunoreactivity of Tuberoinfundibular Dopaminergic Neurons in the Arcuate Nucleus during Pregnancy in the Rat Endocrinology, May 1, 1999; 140(5): 2159 - 2166. [Abstract] [Full Text]

				Y. Sun and M. L. Duckworth Identification of a Placental-Specific Enhancer in the Rat Placental Lactogen II Gene That Contains Binding Sites for Members of the Ets and AP-1 (Activator Protein 1) Families of Transcription Factors Mol. Endocrinol., March 1, 1999; 13(3): 385 - 399. [Abstract] [Full Text]

				A. MacAuley, J. C. Cross, and Z. Werb Reprogramming the Cell Cycle for Endoreduplication in Rodent Trophoblast Cells Mol. Biol. Cell, April 1, 1998; 9(4): 795 - 807. [Abstract] [Full Text]

				J. Lin, J. Poole, and D. I. H. Linzer Two Novel Members of the Prolactin/Growth Hormone Family Are Expressed in the Mouse Placenta Endocrinology, December 1, 1997; 138(12): 5535 - 5540. [Abstract] [Full Text] [PDF]

				K. E. Orwig, G. Dai, C. A. Rasmussen, and M. J. Soares Decidual/Trophoblast Prolactin-Related Protein: Characterization of Gene Structure and Cell-Specific Expression Endocrinology, June 1, 1997; 138(6): 2491 - 2500. [Abstract] [Full Text] [PDF]

				G. Ma, M. Roth, J. Groskopf, F. Tsai, S. Orkin, F Grosveld, J. Engel, and D. Linzer GATA-2 and GATA-3 regulate trophoblast-specific gene expression in vivo Development, January 2, 1997; 124(4): 907 - 914. [Abstract] [PDF]

				J. Cross, M. Flannery, M. Blanar, E Steingrimsson, N. Jenkins, N. Copeland, W. Rutter, and Z Werb Hxt encodes a basic helix-loop-helix transcription factor that regulates trophoblast cell development Development, January 8, 1995; 121(8): 2513 - 2523. [Abstract] [PDF]

				Y. Ng, K. George, J. Engel, and D. Linzer GATA factor activity is required for the trophoblast-specific transcriptional regulation of the mouse placental lactogen I gene Development, January 11, 1994; 120(11): 3257 - 3266. [Abstract] [PDF]