help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fitzpatrick, S. L.
Right arrow Articles by Richards, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fitzpatrick, S. L.
Right arrow Articles by Richards, J. S.

Molecular Endocrinology, Vol 7, 341-354, Copyright © 1993 by Endocrine Society

ARTICLES

cis-acting elements of the rat aromatase promoter required for cyclic adenosine 3',5'-monophosphate induction in ovarian granulosa cells and constitutive expression in R2C Leydig cells

SL Fitzpatrick and JS Richards
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.

The cytochrome P450 aromatase gene is transcriptionally regulated by FSH and steroids in granulosa cells of developing ovarian follicles. To characterize the molecular mechanisms by which this regulation occurs, the promoter of the rat aromatase gene has been analyzed by 1) mapping the transcriptional start site, 2) constructing deletion mutant reporter genes for transfection assays, and 3) determining DNA-protein interactions by gel shift assays. Transient transfection assays indicated that promoter sequences between -176 and -31 basepairs (bp) were required for cAMP inducibility of reporter constructs in primary cultures of granulosa cells and for expression in rat R2C Leydig cells, which constitutively express high amounts of aromatase mRNA. Nuclear extract proteins from granulosa cells and R2C Leydig cells specifically bound a labeled -176/13-bp fragment and were competed by cold competitor fragment as well as by a shorter region (-90/-66 bp) containing an AGGTCA hexameric motif. Competition was inhibited by mutation of the central GGs and was affected by adjacent 5' contextual sequences. A possible candidate for the binding activity observed in granulosa cells and R2C cell nuclear extracts binds an oligonucleotide containing the aromatase AGGTCA motif and is an orphan member of the steroid/thyroid hormone superfamily. These results are the first to characterize cis-acting DNA elements in the aromatase promoter, identify a region that confers cAMP inducibility in granulosa cells and constitutive expression in R2C cells, and localize a hexameric sequence within this region that binds at least one member of the orphan receptor class of transcription factors.


This article has been cited by other articles:


Home page
 J Mol EndocrinolHome page
C. Stocco, J. Kwintkiewicz, and Z. Cai
Identification of regulatory elements in the Cyp19 proximal promoter in rat luteal cells
J. Mol. Endocrinol., October 1, 2007; 39(4): 211 - 221.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Sirianni, A. Chimento, R. Malivindi, I. Mazzitelli, S. Ando, and V. Pezzi
Insulin-Like Growth Factor-I, Regulating Aromatase Expression through Steroidogenic Factor 1, Supports Estrogen-Dependent Tumor Leydig Cell Proliferation
Cancer Res., September 1, 2007; 67(17): 8368 - 8377.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. Kwintkiewicz, Z. Cai, and C. Stocco
Follicle-Stimulating Hormone-Induced Activation of Gata4 Contributes in the Up-Regulation of Cyp19 Expression in Rat Granulosa Cells
Mol. Endocrinol., April 1, 2007; 21(4): 933 - 947.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. H. Mellon, S. R. Bair, C. Depoix, J.-L. Vigne, N. B. Hecht, and P. B. Brake
Translin Coactivates Steroidogenic Factor-1-Stimulated Transcription
Mol. Endocrinol., January 1, 2007; 21(1): 89 - 105.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. Weck and K. E. Mayo
Switching of NR5A Proteins Associated with the Inhibin {alpha}-Subunit Gene Promoter after Activation of the Gene in Granulosa Cells
Mol. Endocrinol., May 1, 2006; 20(5): 1090 - 1103.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
F. X. Donadeu and M. Ascoli
The Differential Effects of the Gonadotropin Receptors on Aromatase Expression in Primary Cultures of Immature Rat Granulosa Cells Are Highly Dependent on the Density of Receptors Expressed and the Activation of the Inositol Phosphate Cascade
Endocrinology, September 1, 2005; 146(9): 3907 - 3916.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M.-C. Chiang, Y.-C. Lee, C.-L. Huang, and Y. Chern
cAMP-response Element-binding Protein Contributes to Suppression of the A2A Adenosine Receptor Promoter by Mutant Huntingtin with Expanded Polyglutamine Residues
J. Biol. Chem., April 8, 2005; 280(14): 14331 - 14340.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
W. Zheng and C. R. Jefcoate
Steroidogenic Factor-1 Interacts with cAMP Response Element-Binding Protein to Mediate cAMP Stimulation of CYP1B1 via a Far Upstream Enhancer
Mol. Pharmacol., February 1, 2005; 67(2): 499 - 512.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Stocco
In Vivo and in Vitro Inhibition of cyp19 Gene Expression by Prostaglandin F2{alpha} in Murine Luteal Cells: Implication of GATA-4
Endocrinology, November 1, 2004; 145(11): 4957 - 4966.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. E. Falender, R. Lanz, D. Malenfant, L. Belanger, and J. S. Richards
Differential Expression of Steroidogenic Factor-1 and FTF/LRH-1 in the Rodent Ovary
Endocrinology, August 1, 2003; 144(8): 3598 - 3610.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. M. Salvador, Y. Park, J. Cottom, E. T. Maizels, J. C. R. Jones, R. V. Schillace, D. W. Carr, P. Cheung, C. D. Allis, J. L. Jameson, et al.
Follicle-stimulating Hormone Stimulates Protein Kinase A-mediated Histone H3 Phosphorylation and Acetylation Leading to Select Gene Activation in Ovarian Granulosa Cells
J. Biol. Chem., October 19, 2001; 276(43): 40146 - 40155.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. Gen, K. Okuzawa, N. Kumakura, S. Yamaguchi, and H. Kagawa
Correlation Between Messenger RNA Expression of Cytochrome P450 Aromatase and Its Enzyme Activity During Oocyte Development in the Red Seabream (Pagrus major)
Biol Reprod, October 1, 2001; 65(4): 1186 - 1194.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. Ito, Y. Park, J. Weck, K. E. Mayo, and J. L. Jameson
Synergistic Activation of the Inhibin {alpha}-Promoter by Steroidogenic Factor-1 and Cyclic Adenosine 3',5'-Monophosphate
Mol. Endocrinol., January 1, 2000; 14(1): 66 - 81.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
K. Kawabe, T. Shikayama, H. Tsuboi, S. Oka, K. Oba, T. Yanase, H. Nawata, and K.-i. Morohashi
Dax-1 as One of the Target Genes of Ad4BP/SF-1
Mol. Endocrinol., August 1, 1999; 13(8): 1267 - 1284.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
T. Hirakawa, T. Minegishi, M. Tano, T. Kameda, H. Kishi, Y. Ibuki, T. Mizutani, and K. Miyamoto
Effect of Prolactin on the Expression of Luteinizing Hormone Receptors during Cell Differentiation in Cultured Rat Granulosa Cells
Endocrinology, August 1, 1999; 140(8): 3444 - 3451.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
E. Silverman, S. Eimerl, and J. Orly
CCAAT Enhancer-binding Protein beta  and GATA-4 Binding Regions within the Promoter of the Steroidogenic Acute Regulatory Protein (StAR) Gene Are Required for Transcription in Rat Ovarian Cells
J. Biol. Chem., June 18, 1999; 274(25): 17987 - 17996.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
W. Yang, P. A. Friedman, R. Kumar, J. L. Omdahl, B. K. May, M.-L. Siu-Caldera, G. S. Reddy, and S. Christakos
Expression of 25(OH)D3 24-hydroxylase in distal nephron: coordinate regulation by 1,25(OH)2D3 and cAMP or PTH
Am J Physiol Endocrinol Metab, April 1, 1999; 276(4): E793 - E805.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Young and M. J. McPhaul
A Steroidogenic Factor-1-Binding Site and Cyclic Adenosine 3',5'-Monophosphate Response Element-Like Elements Are Required for the Activity of the Rat Aromatase Promoter in Rat Leydig Tumor Cell Lines
Endocrinology, December 1, 1998; 139(12): 5082 - 5093.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. Kamat, J. L. Alcorn, C. Kunczt, and C. R. Mendelson
Characterization of the Regulatory Regions of the Human Aromatase (P450arom) Gene Involved in Placenta-Specific Expression
Mol. Endocrinol., November 1, 1998; 12(11): 1764 - 1777.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
D. Monte, F. DeWitte, and D. W. Hum
Regulation of the Human P450scc Gene by Steroidogenic Factor 1 Is Mediated by CBP/p300
J. Biol. Chem., February 20, 1998; 273(8): 4585 - 4591.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
A. Amsterdam and N. Selvaraj
Control of Differentiation, Transformation, and Apoptosis in Granulosa Cells by Oncogenes, Oncoviruses, and Tumor Suppressor Genes
Endocr. Rev., August 1, 1997; 18(4): 435 - 461.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Mayerhofer, G. A. Dissen, M. E. Costa, and S. R. Ojeda
A Role for Neurotransmitters in Early Follicular Development: Induction of Functional Follicle-Stimulating Hormone Receptors in Newly Formed Follicles of the Rat Ovary
Endocrinology, August 1, 1997; 138(8): 3320 - 3329.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. Furbass, C. Kalbe, and J. Vanselow
Tissue-Specific Expression of the Bovine Aromatase-Encoding Gene Uses Multiple Transcriptional Start Sites and Alternative First Exons
Endocrinology, July 1, 1997; 138(7): 2813 - 2819.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
K. L. Parker and B. P. Schimmer
Steroidogenic Factor 1: A Key Determinant of Endocrine Development and Function
Endocr. Rev., June 1, 1997; 18(3): 361 - 377.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
E. Vicini and M. Conti
Characterization of an Intronic Promoter of a Cyclic Adenosine 3',5'-Monophosphate (cAMP)-Specific Phosphodiesterase Gene that Confers Hormone and cAMP Inducibility
Mol. Endocrinol., June 1, 1997; 11(7): 839 - 850.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
D. L. Carlone and J. S. Richards
Functional Interactions, Phosphorylation, and Levels of 3',5'-Cyclic Adenosine Monophosphate-Regulatory Element Binding Protein and Steroidogenic Factor-1 Mediate Hormone-Regulated and Constitutive Expression of Aromatase in Gonadal Cells
Mol. Endocrinol., March 1, 1997; 11(3): 292 - 304.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
G Giuili, W. Shen, and H. Ingraham
The nuclear receptor SF-1 mediates sexually dimorphic expression of Mullerian Inhibiting Substance, in vivo
Development, January 5, 1997; 124(9): 1799 - 1807.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
J. K. Morris and J. S. Richards
An E-box Region within the Prostaglandin Endoperoxide Synthase-2 (PGS-2) Promoter Is Required for Transcription in Rat Ovarian Granulosa Cells
J. Biol. Chem., July 12, 1996; 271(28): 16633 - 16643.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Zhou, P. Clarke, J. Wang, and S. Chen
Identification of a Promoter That Controls Aromatase Expression in Human Breast Cancer and Adipose Stromal Cells
J. Biol. Chem., June 21, 1996; 271(25): 15194 - 15202.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhao, J. E. Nichols, S. E. Bulun, C. R. Mendelson, and E. R. Simpson
Aromatase P450 Gene Expression in Human Adipose Tissue
J. Biol. Chem., July 7, 1995; 270(27): 16449 - 16457.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. D. Michael, M. W. Kilgore, K.-i. Morohashi, and E. R. Simpson
Ad4BP/SF-1 Regulates Cyclic AMP-induced Transcription from the Proximal Promoter (PII) of the Human Aromatase P450 (CYP19) Gene in the Ovary
J. Biol. Chem., June 2, 1995; 270(22): 13561 - 13566.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Ito, J. C. Achermann, and J. L. Jameson
A Naturally Occurring Steroidogenic Factor-1 Mutation Exhibits Differential Binding and Activation of Target Genes
J. Biol. Chem., October 6, 2000; 275(41): 31708 - 31714.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1993 by The Endocrine Society