help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wallis, A. E.
Right arrow Articles by Devlin, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wallis, A. E.
Right arrow Articles by Devlin, R. H.

Molecular Endocrinology, Vol 7, 409-422, Copyright © 1993 by Endocrine Society

ARTICLES

Duplicate insulin-like growth factor-I genes in salmon display alternative splicing pathways

AE Wallis and RH Devlin
Monsanto Canada, Inc., Streetsville, Mississauga, Ontario.

Insulin-like growth factor (IGF-I) is a single chain, 70-amino acid, peptide hormone, which is essential to the process of growth and differentiation in higher vertebrates. IGF-I exhibits a very high degree of conservation throughout vertebrate evolution, and recent studies in fish have shown improved rates of growth in salmonids treated with exogenous recombinant mammalian IGF-I, implying a role for this hormone in fish similar to that in mammals. This paper reports the identification and sequencing of four different liver-derived cDNAs encoding IGF-I from chinook salmon, Oncorhynchus tshawytscha. The different cDNAs all contain sequences that encode an identical mature IGF-I protein. The differences between the four cDNAs are of two types: 1) single basepair changes and 2) additional blocks of sequence occurring at one site within the E-domain regions of three of the four cDNAs. The sequence data indicate that each of the cDNAs identified was transcribed from a different IGF-I gene, and analysis of the chinook salmon genome by Southern blotting supports the existence of four distinct IGF-I genes and suggests that there are at least two IGF-I loci in salmon. Although, analysis of total RNA from salmonid liver identifies only a single major transcript of 4200 nucleotides, polymerase chain reaction analysis suggests the presence of two structurally different salmonid IGF-I genes with the potential for multiple splicing alternatives of the IGF-I transcript.


This article has been cited by other articles:


Home page
 J. Nutr.Home page
C. Duan
Nutritional and Developmental Regulation of Insulin-like Growth Factors in Fish
J. Nutr., February 1, 1998; 128(2): 306 - 306.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
M. Reinecke, A. Schmid, R. Ermatinger, and D. Loffing-Cueni
Insulin-Like Growth Factor I in the Teleost Oreochromis mossambicus, the Tilapia: Gene Sequence, Tissue Expression, and Cellular Localization
Endocrinology, September 1, 1997; 138(9): 3613 - 3619.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1993 by The Endocrine Society