Molecular Endocrinology, Vol 7, 1217-1225, Copyright © 1993 by Endocrine Society

ARTICLES

Identification of 3',5'-cyclic adenosine monophosphate-inducible nuclear factors binding to the human urokinase promoter in mouse Sertoli cells

P Grimaldi, D Piscitelli, C Albanesi, F Blasi, R Geremia and P Rossi
Department of Public Health and Cell Biology, University of Rome Tor Vergata, Italy.

Transcription factors which recognize both the SV40 promoter and the proximal promoter region of the human urokinase-type plasminogen activator (h-uPA) gene are present in nuclear extracts from primary cultures of mouse Sertoli cells; prolonged (more than 12 h) (Bu)2cAMP stimulation of Sertoli cells induces the formation of different specific DNA-protein complexes. A discrete region in the h-uPA promoter, between -54 and -42, is essential for the formation of the cAMP-induced DNA-protein complexes. Mutation of the sequence between - 54 and -42 abolishes the response to cAMP of the proximal h-uPA promoter in Sertoli cells. A protein, recognizing a sequence centered around the GC-box present between -48 and -43, is detected by Southwestern analysis, and it is clearly induced by (Bu)2cAMP stimulation. Interaction between this protein and a second factor, recognizing a purine-rich sequence between -53 and -46, partially overlapping the GC-box, is needed for the formation of the cAMP-induced DNA-protein complexes. A preformed complex between the cAMP-induced GC- box-binding factor and the second factor can be detected using nondenaturing conditions during Southwestern analysis.

This article has been cited by other articles:

				D. Filipponi, R. M. Hobbs, S. Ottolenghi, P. Rossi, E. A. Jannini, P. P. Pandolfi, and S. Dolci Repression of kit Expression by Plzf in Germ Cells Mol. Cell. Biol., October 1, 2007; 27(19): 6770 - 6781. [Abstract] [Full Text] [PDF]

				P. Grimaldi, F. Capolunghi, R. Geremia, and P. Rossi Cyclic Adenosine Monophosphate (cAMP) Stimulation of the Kit Ligand Promoter in Sertoli Cells Requires an Sp1-Binding Region, a Canonical TATA Box, and a cAMP-Induced Factor Binding to an Immediately Downstream GC-Rich Element Biol Reprod, December 1, 2003; 69(6): 1979 - 1988. [Abstract] [Full Text] [PDF]

				M. Pellegrini, P. Grimaldi, P. Rossi, R. Geremia, and S. Dolci Developmental expression of BMP4/ALK3/SMAD5 signaling pathway in the mouse testis: a potential role of BMP4 in spermatogonia differentiation J. Cell Sci., August 15, 2003; 116(16): 3363 - 3372. [Abstract] [Full Text] [PDF]

				S. Chen, X. Liu, and D. L. Segaloff A Novel Cyclic Adenosine 3',5'-Monophosphate-Responsive Element Involved In the Transcriptional Regulation of the Lutropin Receptor Gene in Granulosa Cells Mol. Endocrinol., September 1, 2000; 14(9): 1498 - 1508. [Abstract] [Full Text]

				S. Wang, W. Wang, R. A. Wesley, and R. L. Danner A Sp1 Binding Site of the Tumor Necrosis Factor alpha Promoter Functions as a Nitric Oxide Response Element J. Biol. Chem., November 19, 1999; 274(47): 33190 - 33193. [Abstract] [Full Text] [PDF]

				C. Sette, M. Barchi, A. Bianchini, M. Conti, P. Rossi, and R. Geremia Activation of the Mitogen-activated Protein Kinase ERK1 during Meiotic Progression of Mouse Pachytene Spermatocytes J. Biol. Chem., November 19, 1999; 274(47): 33571 - 33579. [Abstract] [Full Text] [PDF]

				M. Hocker, R. Raychowdhury, T. Plath, H. Wu, D. T. O'Connor, B. Wiedenmann, S. Rosewicz, and T. C. Wang Sp1 and CREB Mediate Gastrin-dependent Regulation of Chromogranin A Promoter Activity in Gastric Carcinoma Cells J. Biol. Chem., December 18, 1998; 273(51): 34000 - 34007. [Abstract] [Full Text] [PDF]

				E. Vicini and M. Conti Characterization of an Intronic Promoter of a Cyclic Adenosine 3',5'-Monophosphate (cAMP)-Specific Phosphodiesterase Gene that Confers Hormone and cAMP Inducibility Mol. Endocrinol., June 1, 1997; 11(7): 839 - 850. [Abstract] [Full Text]

				B. Liu, R. S. Maul, and D. M. Kaetzel Jr. Repression of Platelet-derived Growth Factor A-chain Gene Transcription by an Upstream Silencer Element. PARTICIPATION BY SEQUENCE-SPECIFIC SINGLE-STRANDED DNA-BINDING PROTEINS J. Biol. Chem., October 18, 1996; 271(42): 26281 - 26290. [Abstract] [Full Text] [PDF]

				C Albanesi, R Geremia, M Giorgio, S Dolci, C Sette, and P Rossi A cell- and developmental stage-specific promoter drives the expression of a truncated c-kit protein during mouse spermatid elongation Development, January 4, 1996; 122(4): 1291 - 1302. [Abstract] [PDF]

				P. Venepally and M. R. Waterman Two Sp1-binding Sites Mediate cAMP-induced Transcription of the Bovine CYP11A Gene through the Protein Kinase A Signaling Pathway J. Biol. Chem., October 27, 1995; 270(43): 25402 - 25410. [Abstract] [Full Text] [PDF]