| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Molecular Endocrinology, Vol 8, 1593-1604, Copyright © 1994 by Endocrine Society
ARTICLES |
M Liu and LP Freedman
Department of Pharmacology, Cornell University Graduate School of Medical Sciences, New York, New York 10021.
Small changes in the concentrations and/or combinations of trans-acting factors can result in profound alterations in gene expression. Synergistic interaction between different classes of transcription factors bound to distinct sites within a promoter/enhancer region is one mechanism by which this can occur. Reflecting this, hormone response elements, DNA recognition sites for steroid/nuclear receptors, are often found in promoter regions organized as multiple copies or are clustered among binding sites for other trans-acting factors. To systematically examine the potential interactions between one such receptor, the vitamin D3 receptor (VDR), and other nonreceptor transcription factors, we constructed a series of reporter plasmids containing one copy of the osteopontin (Spp1) vitamin D response element (VDRE), consisting of two direct repeats spaced by 3 base pairs, and one binding site for the transcription factors SP1, NF-1, Oct-1, or AP-1. We also generated reporters either under the control of two copies of Spp1 VDRE, or a distinct VDRE from the human osteocalcin gene promoter. The various reporters were used to transiently transfect HeLa or CV-1 cells in the presence and absence of 1,25-dihydroxyvitamin D3. Our results show that VDR transactivates 12-20 times more strongly from two Spp1-VDREs than from one, indicating that VDR synergizes with itself. VDR also synergizes with the other nonreceptor factors, since we observe a 6- to 12-fold degree of synergistic induction after ligand addition, depending on the particular factor. The functional basis for the transcriptional synergism appears to be at the level of cooperative DNA binding, at least for VDR alone and VDR-Oct-1, as demonstrated in vitro by gel mobility shift assays using purified factors. Consistent with this, we show that the minimal requirement for transcriptional synergism in vivo by VDR is its DNA-binding domain.
This article has been cited by other articles:
 |
|
 |
|
  S. Seoane, I. Ben, V. Centeno, and R. Perez-Fernandez Cellular Expression Levels of the Vitamin D Receptor Are Critical to Its Transcriptional Regulation by the Pituitary Transcription Factor Pit-1 Mol. Endocrinol., July 1, 2007; 21(7): 1513 - 1525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R McGaffin and S. A Chrysogelos Identification and characterization of a response element in the EGFR promoter that mediates transcriptional repression by 1,25-dihydroxyvitamin D3 in breast cancer cells J. Mol. Endocrinol., August 1, 2005; 35(1): 117 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Schneider, C. El-Yazidi, A. Dace, M. Maraninchi, R. Planells, A. Margotat, and J. Torresani Expression of the 1,25-(OH)2 vitamin D3 receptor gene during the differentiation of mouse Ob17 preadipocytes and cross talk with the thyroid hormone receptor signalling pathway J. Mol. Endocrinol., February 1, 2005; 34(1): 221 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yasunami, H. Hara, T. Iwamura, T. Kataoka, and T. Adachi C-JUN N-TERMINAL KINASE MODULATES 1,25-DIHYDROXYVITAMIN D3-INDUCED CYTOCHROME P450 3A4 GENE EXPRESSION Drug Metab. Dispos., July 1, 2004; 32(7): 685 - 688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Matsubara, Y. Wada, T. A. Gardner, M. Egawa, M.-S. Park, C.-L. Hsieh, H. E. Zhau, C. Kao, S. Kamidono, J. Y. Gillenwater, et al. A Conditional Replication-competent Adenoviral Vector, Ad-OC-E1a, to Cotarget Prostate Cancer and Bone Stroma in an Experimental Model of Androgen-independent Prostate Cancer Bone Metastasis Cancer Res., August 1, 2001; 61(16): 6012 - 6019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Andersen and M. G. Rosenfeld POU Domain Factors in the Neuroendocrine System: Lessons from Developmental Biology Provide Insights into Human Disease Endocr. Rev., February 1, 2001; 22(1): 2 - 35. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Ishii, A. J. Hansen, and P. I. Mackenzie Octamer Transcription Factor-1 Enhances Hepatic Nuclear Factor-1alpha -Mediated Activation of the Human UDP Glucuronosyltransferase 2B7 Promoter Mol. Pharmacol., May 1, 2000; 57(5): 940 - 947. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. P. Dwivedi, J. L. Omdahl, I. Kola, D. A. Hume, and B. K. May Regulation of Rat Cytochrome P450C24 (CYP24) Gene Expression. EVIDENCE FOR FUNCTIONAL COOPERATION OF Ras-ACTIVATED Ets TRANSCRIPTION FACTORS WITH THE VITAMIN D RECEPTOR IN 1,25-DIHYDROXYVITAMIN D3-MEDIATED INDUCTION J. Biol. Chem., January 7, 2000; 275(1): 47 - 55. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Blutt, T. J. McDonnell, T. C. Polek, and N. L. Weigel Calcitriol-Induced Apoptosis in LNCaP Cells Is Blocked By Overexpression of Bcl-2 Endocrinology, January 1, 2000; 141(1): 10 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Hmama, D. Nandan, L. Sly, K. L. Knutson, P. Herrera-Velit, and N. E. Reiner 1{alpha},25-Dihydroxyvitamin D3-induced Myeloid Cell Differentiation Is Regulated by a Vitamin D Receptor-Phosphatidylinositol 3-Kinase Signaling Complex J. Exp. Med., December 6, 1999; 190(11): 1583 - 1594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. I. Castillo, A. M. Jimenez-Lara, R. M. Tolon, and A. Aranda Synergistic Activation of the Prolactin Promoter by Vitamin D Receptor and GHF-1: Role of the Coactivators, CREB-Binding Protein and Steroid Hormone Receptor Coactivator-1 (SRC-1) Mol. Endocrinol., July 1, 1999; 13(7): 1141 - 1154. [Abstract] [Full Text]
|
|
|
|
|
|
U. R. Chandran, B. S. Warren, C. T. Baumann, G. L. Hager, and D. B. DeFranco The Glucocorticoid Receptor Is Tethered to DNA-bound Oct-1 at the Mouse Gonadotropin-releasing Hormone Distal Negative Glucocorticoid Response Element J. Biol. Chem., January 22, 1999; 274(4): 2372 - 2378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Grisaru, E. Lev-Lehman, M. Shapira, E. Chaikin, J. B. Lessing, A. Eldor, F. Eckstein, and H. Soreq Human Osteogenesis Involves Differentiation-Dependent Increases in the Morphogenically Active 3' Alternative Splicing Variant of Acetylcholinesterase Mol. Cell. Biol., January 1, 1999; 19(1): 788 - 795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Aslam, L. McCabe, B. Frenkel, A. J. van Wijnen, G. S. Stein, J. B. Lian, and J. L. Stein AP-1 and Vitamin D Receptor (VDR) Signaling Pathways Converge at the Rat Osteocalcin VDR Element: Requirement for the Internal Activating Protein-1 Site for Vitamin D-Mediated Trans-Activation Endocrinology, January 1, 1999; 140(1): 63 - 70. [Abstract] [Full Text]
|
|
|
|
|
|
M. Raval-Pandya, L. P. Freedman, H. Li, and S. Christakos Thyroid Hormone Receptor Does Not Heterodimerize with the Vitamin D Receptor but Represses Vitamin D Receptor-Mediated Transactivation Mol. Endocrinol., September 1, 1998; 12(9): 1367 - 1379. [Abstract] [Full Text]
|
|
|
|
|
|
T. Nishishita, T. Okazaki, T. Ishikawa, T. Igarashi, K. Hata, E. Ogata, and T. Fujita A Negative Vitamin D Response DNA Element in the Human Parathyroid Hormone-related Peptide Gene Binds to Vitamin D Receptor Along with Ku Antigen to Mediate Negative Gene Regulation by Vitamin D J. Biol. Chem., May 1, 1998; 273(18): 10901 - 10907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Liu, W. Ning, R. Dantzer, G. G. Freund, and K. W. Kelley Activation of Protein Kinase C-{zeta} and Phosphatidylinositol 3'-Kinase and Promotion of Macrophage Differentiation by Insulin-Like Growth Factor-I J. Immunol., February 1, 1998; 160(3): 1393 - 1401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Takeuchi, G. S. Reddy, T. Kobayashi, T. Okano, J. Park, and S. Sharma Nuclear Factor of Activated T Cells (NFAT) as a Molecular Target for 1{alpha},25-Dihydroxyvitamin D3-Mediated Effects J. Immunol., January 1, 1998; 160(1): 209 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Porter, B. Saville, D. Hoivik, and S. Safe Functional Synergy between the Transcription Factor Sp1 and the Estrogen Receptor Mol. Endocrinol., October 1, 1997; 11(11): 1569 - 1580. [Abstract] [Full Text]
|
|
|
|
|
|
Z. Xie and D. D. Bikle Cloning of the Human Phospholipase C-gamma 1 Promoter and Identification of a DR6-type Vitamin D-responsive Element J. Biol. Chem., March 7, 1997; 272(10): 6573 - 6577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. B. Sneddon, C. E. Bogado, M. S. Kiernan, and M. B. Demay DNA Sequences Downstream from the Vitamin D Response Element of the Rat Osteocalcin Gene Are Required for Ligand-Dependent Transactivation Mol. Endocrinol., February 1, 1997; 11(2): 210 - 217. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Ohyama, K. Ozono, M. Uchida, M. Yoshimura, T. Shinki, T. Suda, and O. Yamamoto Functional Assessment of Two Vitamin D-responsive Elements in the Rat 25-Hydroxyvitamin D3 24-Hydroxylase Gene J. Biol. Chem., November 29, 1996; 271(48): 30381 - 30385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Kerry, P. P. Dwivedi, C. N. Hahn, H. A. Morris, J. L. Omdahl, and B. K. May Transcriptional Synergism between Vitamin D-responsive Elements in the Rat 25-Hydroxyvitamin D3 24-Hydroxylase (CYP24) Promoter J. Biol. Chem., November 22, 1996; 271(47): 29715 - 29721. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
