| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Molecular Endocrinology, Vol 8, 148-158, Copyright © 1994 by Endocrine Society
ARTICLES |
H Weber, ML Webb, R Serafino, DS Taylor, S Moreland, J Norman and CJ Molloy
Department of Cardiovascular Biochemistry, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543.
The vasoactive peptides endothelin-1 (ET-1) and angiotensin-II (AII) have been implicated in chronic hypertension and may play important roles in related vascular diseases such as restenosis and atherosclerosis. Using a rat aortic smooth muscle (RASM) cell model, both ET-1 and AII induced concentration-dependent delayed increases in DNA synthesis relative to that in the serum-deprived controls. Stimulation of DNA synthesis was maximal at 100 nM for each peptide. All treatment of RASM cells resulted in a greater mitogenic effect (4- to 7-fold) than that observed for ET-1 (3-fold). When added in the presence of AII, ET-1 had a supplemental effect on DNA synthesis (5- to 10-fold above control). Although RASM cells expressed both ETA and AT1 receptors, radioligand binding experiments indicated that approximately 10-fold as many AT1 receptors as ETA receptors were present. In signal transduction studies, ET-1 and AII each elicited concentration- dependent increases in the intracellular Ca2+ concentration. ET-1 and AII also stimulated phosphoinositide metabolism and phosphorylation of a specific substrate for protein kinase-C. The release of total inositol phosphates in response to ET-1 and AII was concentration dependent and inhibited by the ETA receptor-selective antagonist BQ-123 and the AT1 receptor-selective antagonist losartan, respectively. In addition, tyrosine phosphorylation of 120- and 75-kilodalton proteins as well as the mitogen-activated protein kinases p44mapk and p42mapk was observed within 5 min of the addition of either ET-1 or AII. Taken together, these data indicate that ET-1 and AII may promote smooth muscle cell growth through common intracellular signaling mechanisms.
This article has been cited by other articles:
 |
|
 |
|
  F. Xiao, J. R Puddefoot, S. Barker, and G. P Vinson Changes in angiotensin II type 1 receptor signalling pathways evoked by a monoclonal antibody raised to the N-terminus J. Endocrinol., April 1, 2008; 197(1): 25 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Wolf, G. Sauter, H.-P. Rodemann, T. Risler, and B. R. Brehm Influence of growth factors on the proliferation of vascular smooth muscle cells isolated from subtotally nephrectomized rats after endothelin or angiotensin II antagonism Nephrol. Dial. Transplant., February 1, 2005; 20(2): 312 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Tallant and M. A. Clark Molecular Mechanisms of Inhibition of Vascular Growth by Angiotensin-(1-7) Hypertension, October 1, 2003; 42(4): 574 - 579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W Barolet, S. Babaei, R. Robinson, P. Picard, W. Tsui, N. Nili, F. Mohamed, O. Ornatsky, J. D Sparkes, D. J Stewart, et al. Administration of exogenous endothelin-1 following vascular balloon injury: early and late effects on intimal hyperplasia Cardiovasc Res, December 1, 2001; 52(3): 468 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Guillemot, A. Levy, M. Raymondjean, and B. Rothhut Angiotensin II-induced Transcriptional Activation of the Cyclin D1 Gene Is Mediated by Egr-1 in CHO-AT1A Cells J. Biol. Chem., October 12, 2001; 276(42): 39394 - 39403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Berk Vascular Smooth Muscle Growth: Autocrine Growth Mechanisms Physiol Rev, July 1, 2001; 81(3): 999 - 1030. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y Hojo, U Ikeda, T Katsuki, O Mizuno, H Fujikawa, and K Shimada Inhibition of angiotensin converting enzyme cannot prevent increases in angiotensin II production in coronary circulation Heart, May 1, 2000; 83(5): 574 - 576. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. P. Rossi, A. Sacchetto, D. Rizzoni, S. Bova, E. Porteri, G. Mazzocchi, A. S. Belloni, M. Bahcelioglu, G. G. Nussdorfer, and A. C. Pessina Blockade of Angiotensin II Type 1 Receptor and Not of Endothelin Receptor Prevents Hypertension and Cardiovascular Disease in Transgenic (mREN2)27 Rats via Adrenocortical Steroid-Independent Mechanisms Arterioscler. Thromb. Vasc. Biol., April 1, 2000; 20(4): 949 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Damon VSM growth is stimulated in sympathetic neuron/VSM cocultures: role of TGF-beta 2 and endothelin Am J Physiol Heart Circ Physiol, February 1, 2000; 278(2): H404 - H411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Tallant, D. I. Diz, and C. M. Ferrario Antiproliferative Actions of Angiotensin-(1-7) in Vascular Smooth Muscle Hypertension, October 1, 1999; 34(4): 950 - 957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Barnes and A. J Turner Endothelin converting enzyme is located on {alpha}-actin filaments in smooth muscle cells Cardiovasc Res, June 1, 1999; 42(3): 814 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Taylor, X. Cheng, J. E. Pawlowski, A. R. Wallace, P. Ferrer, and C. J. Molloy Epiregulin is a potent vascular smooth muscle cell-derived mitogen induced by angiotensin II, endothelin-1, and thrombin PNAS, February 16, 1999; 96(4): 1633 - 1638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Suenobu, M. Shichiri, M. Iwashina, F. Marumo, and Y. Hirata Natriuretic Peptides and Nitric Oxide Induce Endothelial Apoptosis via a cGMP–Dependent Mechanism Arterioscler. Thromb. Vasc. Biol., January 1, 1999; 19(1): 140 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. TÖRNIG, M.-L. GROSS, A. SIMONAVICIENE, G. MALL, E. RITZ, and K. AMANN Hypertrophy of Intramyocardial Arteriolar Smooth Muscle Cells in Experimental Renal Failure J. Am. Soc. Nephrol., January 1, 1999; 10(1): 77 - 83. [Abstract] [Full Text]
|
|
|
|
|
|
B. C. Yang, M. I. Phillips, D. Mohuczy, H. Meng, L. Shen, P. Mehta, and J. L. Mehta Increased Angiotensin II Type 1 Receptor Expression in Hypercholesterolemic Atherosclerosis in Rabbits Arterioscler. Thromb. Vasc. Biol., September 1, 1998; 18(9): 1433 - 1439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kirchengast and K. Munter Endothelin and restenosis Cardiovasc Res, September 1, 1998; 39(3): 550 - 555. [Full Text] [PDF]
|
|
|
|
|
|
T. Tonnessen, P. Kristian Lunde, A. Giaid, O. M Sejersted, and G. Christensen Pulmonary and cardiac expression of preproendothelin-1 mRNA are increased in heart failure after myocardial infarction in rats. Localization of preproendothelin-1 mRNA and endothelin peptide Cardiovasc Res, September 1, 1998; 39(3): 633 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Su, D. M. Lombardi, J. Wiener, M. J. A. P. Daemen, M. A. Reidy, and S. M. Schwartz Mitogenic Effect of Angiotensin II on Rat Carotid Arteries and Type II or III Mesenteric Microvessels but Not Type I Mesenteric Microvessels Is Mediated by Endogenous Basic Fibroblast Growth Factor Circ. Res., February 23, 1998; 82(3): 321 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Freeman, M. L. Ruehr, and R. V. Dorman ANG II-induced translocation of cytosolic PLA2 to the nucleus in vascular smooth muscle cells Am J Physiol Cell Physiol, January 1, 1998; 274(1): C282 - C288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-P. Sung, A. J. Arleth, C. Eichman, A. Truneh, and E. H. Ohlstein Carvedilol, a Multiple-Action Neurohumoral Antagonist, Inhibits Mitogen-Activated Protein Kinase and Cell Cycle Progression in Vascular Smooth Muscle Cells J. Pharmacol. Exp. Ther., November 1, 1997; 283(2): 910 - 917. [Abstract] [Full Text]
|
|
|
|
|
|
T. Takizawa, M. Gu, A. V. Chobanian, and P. Brecher Effect of Nitric Oxide on DNA Replication Induced by Angiotensin II in Rat Cardiac Fibroblasts Hypertension, November 1, 1997; 30(5): 1035 - 1040. [Abstract] [Full Text]
|
|
|
|
|
|
J. D. Banting, K. E. Thompson, P. Friberg, and M. A. Adams Blunted Cardiovascular Growth Induction During Prolonged Nitric Oxide Synthase Blockade Hypertension, September 1, 1997; 30(3): 416 - 421. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Hasdai, E. Erez, I. Gil-Ad, E. Raanani, S. Sclarovsky, Y. Barak, J. Sulkes, and B. A. Vidne IS THE HEART A SOURCE FOR ELEVATED CIRCULATING ENDOTHELIN LEVELS DURING AORTA--CORONARY ARTERY BYPASS GRAFTING SURGERY IN HUMAN BEINGS? J. Thorac. Cardiovasc. Surg., August 1, 1996; 112(2): 531 - 536. [Abstract] [Full Text]
|
|
|
|
|
|
E. J. Freeman, G. M. Chisolm, C. M. Ferrario, and E. A. Tallant Angiotensin-(1-7) Inhibits Vascular Smooth Muscle Cell Growth Hypertension, July 1, 1996; 28(1): 104 - 108. [Abstract] [Full Text]
|
|
|
|
|
|
G. Wolf, F. N. Ziyadeh, G. Zahner, and R. A.K. Stahl Angiotensin II Is Mitogenic for Cultured Rat Glomerular Endothelial Cells Hypertension, April 1, 1996; 27(4): 897 - 905. [Abstract] [Full Text]
|
|
|
|
|
|
C. E. Whitworth, M. M. Veniant, J. D. Firth, A. D. Cumming, and J. J. Mullins Endothelin in the Kidney in Malignant Phase Hypertension Hypertension, December 1, 1995; 26(6): 925 - 931. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Turner, K. Pietras, D. Taylor, and C. Molloy Angiotensin II stimulation of rapid paxillin tyrosine phosphorylation correlates with the formation of focal adhesions in rat aortic smooth muscle cells J. Cell Sci., January 1, 1995; 108(1): 333 - 342. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
