help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Clevenger, C. V.
Right arrow Articles by Medaglia, M. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Clevenger, C. V.
Right arrow Articles by Medaglia, M. V.

Molecular Endocrinology, Vol 8, 674-681, Copyright © 1994 by Endocrine Society

ARTICLES

The protein tyrosine kinase P59fyn is associated with prolactin (PRL) receptor and is activated by PRL stimulation of T-lymphocytes

CV Clevenger and MV Medaglia
Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia 19104.

The clonal expansion of antigen-stimulated T-lymphocytes during an immune response is mediated by several lymphokines. Strong evidence now exists that the neuroendocrine hormone PRL is necessary, but not sufficient, for T-cell proliferation. Little is known, however, of the signal transduction mechanisms of the PRL receptor (PRLR) within T- cells. We demonstrate here that PRL stimulation of the T-cell line Nb2 induced the concentration- and time-dependent activation of the protein tyrosine kinase p59fyn, but not of four other src family protein tyrosine kinases. Activation of fyn was also observed in Concanavalin-A- primed peripheral blood lymphocytes stimulated with PRL and in Nb2 cells incubated with anti-PRLR antibodies. The activation of fyn by PRL stimulation correlated with Nb2 cell proliferation. Immunoblot analysis of anti-fyn and anti-PRLR immune complexes revealed an association between each PRLR isoform and p59fyn. These studies demonstrate for the first time an association between the PRLR and a src family protein tyrosine kinase affiliated with signal transduction.


This article has been cited by other articles:


Home page
 J Mol EndocrinolHome page
J. E Morabito, J. F Trott, D. M Korz, H. E Fairfield, S. H Buck, and R. C Hovey
A 5' distal palindrome within the mouse mammary tumor virus-long terminal repeat recruits a mammary gland-specific complex and is required for a synergistic response to progesterone plus prolactin
J. Mol. Endocrinol., August 1, 2008; 41(2): 75 - 90.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
L. M. Neilson, J. Zhu, J. Xie, M. G. Malabarba, K. Sakamoto, K.-U. Wagner, R. A. Kirken, and H. Rui
Coactivation of Janus Tyrosine Kinase (Jak)1 Positively Modulates Prolactin-Jak2 Signaling in Breast Cancer: Recruitment of ERK and Signal Transducer and Activator of Transcription (Stat)3 and Enhancement of Akt and Stat5a/b Pathways
Mol. Endocrinol., September 1, 2007; 21(9): 2218 - 2232.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. J. LeBaron, T. J. Ahonen, M. T. Nevalainen, and H. Rui
In Vivo Response-Based Identification of Direct Hormone Target Cell Populations Using High-Density Tissue Arrays
Endocrinology, March 1, 2007; 148(3): 989 - 1008.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. L. Gadd and C. V. Clevenger
Ligand-Independent Dimerization of the Human Prolactin Receptor Isoforms: Functional Implications
Mol. Endocrinol., November 1, 2006; 20(11): 2734 - 2746.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K.-U. Wagner, A. Krempler, A. A. Triplett, Y. Qi, N. M. George, J. Zhu, and H. Rui
Impaired Alveologenesis and Maintenance of Secretory Mammary Epithelial Cells in Jak2 Conditional Knockout Mice
Mol. Cell. Biol., June 15, 2004; 24(12): 5510 - 5520.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. J. Acosta, R. M. Munoz, L. Gonzalez, A. Subtil-Rodriguez, M. A. Dominguez-Caceres, J. M. Garcia-Martinez, A. Calcabrini, I. Lazaro-Trueba, and J. Martin-Perez
Src Mediates Prolactin-Dependent Proliferation of T47D and MCF7 Cells via the Activation of Focal Adhesion Kinase/Erk1/2 and Phosphatidylinositol 3-Kinase Pathways
Mol. Endocrinol., November 1, 2003; 17(11): 2268 - 2282.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. A. Olayioye, M. A. Guthridge, F. C. Stomski, A. F. Lopez, J. E. Visvader, and G. J. Lindeman
Threonine 391 Phosphorylation of the Human Prolactin Receptor Mediates a Novel Interaction with 14-3-3 Proteins
J. Biol. Chem., August 29, 2003; 278(35): 32929 - 32935.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
C. V. Clevenger, P. A. Furth, S. E. Hankinson, and L. A. Schuler
The Role of Prolactin in Mammary Carcinoma
Endocr. Rev., February 1, 2003; 24(1): 1 - 27.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. B. Kline, M. A. Rycyzyn, and C. V. Clevenger
Characterization of a Novel and Functional Human Prolactin Receptor Isoform ({Delta}S1PRLr) Containing Only One Extracellular Fibronectin-Like Domain
Mol. Endocrinol., October 1, 2002; 16(10): 2310 - 2322.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Chughtai, S. Schimchowitsch, J.-J. Lebrun, and S. Ali
Prolactin Induces SHP-2 Association with Stat5, Nuclear Translocation, and Binding to the beta -Casein Gene Promoter in Mammary Cells
J. Biol. Chem., August 16, 2002; 277(34): 31107 - 31114.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
I. Y. H. Mak, J. J. Brosens, M. Christian, F. A. Hills, L. Chamley, L. Regan, and J. O. White
Regulated Expression of Signal Transducer and Activator of Transcription, Stat5, and its Enhancement of PRL Expression in Human Endometrial Stromal Cells in Vitro
J. Clin. Endocrinol. Metab., June 1, 2002; 87(6): 2581 - 2588.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. A. Rycyzyn and C. V. Clevenger
The intranuclear prolactin/cyclophilin B complex as a transcriptional inducer
PNAS, May 14, 2002; 99(10): 6790 - 6795.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
O. Gubbay, H. O. D. Critchley, J. M. Bowen, A. King, and H. N. Jabbour
Prolactin Induces ERK Phosphorylation in Epithelial and CD56+ Natural Killer Cells of the Human Endometrium
J. Clin. Endocrinol. Metab., May 1, 2002; 87(5): 2329 - 2335.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Xie, M. J. LeBaron, M. T. Nevalainen, and H. Rui
Role of Tyrosine Kinase Jak2 in Prolactin-induced Differentiation and Growth of Mammary Epithelial Cells
J. Biol. Chem., April 12, 2002; 277(16): 14020 - 14030.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. L. Brockman, M. D. Schroeder, and L. A. Schuler
PRL Activates the Cyclin D1 Promoter Via the Jak2/Stat Pathway
Mol. Endocrinol., April 1, 2002; 16(4): 774 - 784.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. Frasor, K. Park, M. Byers, C. Telleria, T. Kitamura, L.-y. Yu-Lee, J. Djiane, O.-K. Park-Sarge, and G. Gibori
Differential Roles for Signal Transducers and Activators of Transcription 5a and 5b in PRL Stimulation of ER{alpha} and ER{beta} Transcription
Mol. Endocrinol., December 1, 2001; 15(12): 2172 - 2181.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. Frasor, U. Barkai, L. Zhong, A. T. Fazleabas, and G. Gibori
PRL-Induced ER{alpha} Gene Expression Is Mediated by Janus Kinase 2 (Jak2) While Signal Transducer and Activator of Transcription 5b (Stat5b) Phosphorylation Involves Jak2 and a Second Tyrosine Kinase
Mol. Endocrinol., November 1, 2001; 15(11): 1941 - 1952.
[Abstract] [Full Text] [PDF]

Home page
 LupusHome page
C V Clevenger and J B Kline
Prolactin receptor signal transduction
Lupus, October 1, 2001; 10(10): 706 - 718.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
D. E. Fleenor and M. Freemark
Prolactin Induction of Insulin Gene Transcription: Roles of Glucose and Signal Transducer and Activator of Transcription 5
Endocrinology, July 1, 2001; 142(7): 2805 - 2810.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. A. F. Vara, M. A. D. Caceres, A. Silva, and J. Martin-Perez
Src Family Kinases Are Required for Prolactin Induction of Cell Proliferation
Mol. Biol. Cell, July 1, 2001; 12(7): 2171 - 2183.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. B. Kline, D. J. Moore, and C. V. Clevenger
Activation and Association of the Tec Tyrosine Kinase with the Human Prolactin Receptor: Mapping of a Tec/Vav1-Receptor Binding Site
Mol. Endocrinol., May 1, 2001; 15(5): 832 - 841.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
N. G. Selvaraj, E. Omi, G. Gibori, and M. C. Rao
Janus Kinase 2 (JAK2) Regulates Prolactin-Mediated Chloride Transport in Mouse Mammary Epithelial Cells through Tyrosine Phosphorylation of Na+-K+-2Cl- Cotransporter
Mol. Endocrinol., December 1, 2000; 14(12): 2054 - 2065.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
M. A. Rycyzyn, S. C. Reilly, K. O’Malley, and C. V. Clevenger
Role of Cyclophilin B in Prolactin Signal Transduction and Nuclear Retrotranslocation
Mol. Endocrinol., August 1, 2000; 14(8): 1175 - 1186.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
I. Olazabal, J. Muñoz, S. Ogueta, E. Obregón, and J. P. García-Ruiz
Prolactin (PRL)-PRL Receptor System Increases Cell Proliferation Involving JNK (c-Jun Amino Terminal Kinase) and AP-1 Activation: Inhibition by Glucocorticoids
Mol. Endocrinol., April 1, 2000; 14(4): 564 - 575.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
K. M. McAveney, M. L. Book, P. Ling, J. Chebath, and L.-y. Yu-Lee
Association of 2',5'-Oligoadenylate Synthetase with the Prolactin (PRL) Receptor: Alteration in PRL-Inducible Stat1 (Signal Transducer and Activator of Transcription 1) Signaling to the IRF-1 (Interferon-Regulatory Factor 1) Promoter
Mol. Endocrinol., February 1, 2000; 14(2): 295 - 306.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. B. Kline, H. Roehrs, and C. V. Clevenger
Functional Characterization of the Intermediate Isoform of the Human Prolactin Receptor
J. Biol. Chem., December 10, 1999; 274(50): 35461 - 35468.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
D. L. Russell and J. S. Richards
Differentiation-Dependent Prolactin Responsiveness and Stat (Signal Transducers and Activators of Transcription) Signaling in Rat Ovarian Cells
Mol. Endocrinol., December 1, 1999; 13(12): 2049 - 2064.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
K. E. Borg, M. Zhang, D. Hegge, R. L. Stephen, D. J. Buckley, N. S. Magnuson, and A. R. Buckley
Prolactin Regulation of pim-1 Expression: Positive and Negative Promoter Elements
Endocrinology, December 1, 1999; 140(12): 5659 - 5668.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
D. Coss, C. B. Kuo, L. Yang, P. Ingleton, R. Luben, and A. M. Walker
Dissociation of Janus Kinase 2 and Signal Transducer and Activator of Transcription 5 Activation after Treatment of Nb2 Cells with a Molecular Mimic of Phosphorylated Prolactin
Endocrinology, November 1, 1999; 140(11): 5087 - 5094.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
B. Tsunekawa, M. Wada, M. Ikeda, H. Uchida, N. Naito, and M. Honjo
The 20-Kilodalton (kDa) Human Growth Hormone (hGH) Differs from the 22-kDa hGH in the Effect on the Human Prolactin Receptor
Endocrinology, September 1, 1999; 140(9): 3909 - 3918.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
A. V. Kazansky, E. B. Kabotyanski, S. L. Wyszomierski, M. A. Mancini, and J. M. Rosen
Differential Effects of Prolactin and src/abl Kinases on the Nuclear Translocation of STAT5B and STAT5A
J. Biol. Chem., August 6, 1999; 274(32): 22484 - 22492.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Yamashita, J. Xu, R. A. Erwin, A. C. Larner, and H. Rui
A Lymphoma Growth Inhibitor Blocks Some but Not All Prolactin-stimulated Signaling Pathways
J. Biol. Chem., May 21, 1999; 274(21): 14699 - 14705.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. C. H. Lee, J. A. Walters, M. E. Reyland, and S. M. Anderson
Constitutive Activation of the Prolactin Receptor Results in the Induction of Growth Factor-independent Proliferation and Constitutive Activation of Signaling Molecules
J. Biol. Chem., April 9, 1999; 274(15): 10024 - 10034.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Hunter, E. A. Burton, S. C. Wu, and S. M. Anderson
Fyn Associates with Cbl and Phosphorylates Tyrosine 731 in Cbl, A Binding Site for Phosphatidylinositol 3-Kinase
J. Biol. Chem., January 22, 1999; 274(4): 2097 - 2106.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. J. Corey and S. M. Anderson
Src-Related Protein Tyrosine Kinases in Hematopoiesis
Blood, January 1, 1999; 93(1): 1 - 14.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Zhu, E. L. K. Goh, D. LeRoith, and P. E. Lobie
Growth Hormone Stimulates the Formation of a Multiprotein Signaling Complex Involving p130Cas and CrkII. RESULTANT ACTIVATION OF c-Jun N-TERMINAL KINASE/STRESS-ACTIVATED PROTEIN KINASE (JNK/SAPK)
J. Biol. Chem., December 11, 1998; 273(50): 33864 - 33875.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Moutoussamy, F. Renaudie, F. Lago, P. A. Kelly, and J. Finidori
Grb10 Identified as a Potential Regulator of Growth Hormone (GH) Signaling by Cloning of GH Receptor Target Proteins
J. Biol. Chem., June 26, 1998; 273(26): 15906 - 15912.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
C. Bole-Feysot, V. Goffin, M. Edery, N. Binart, and P. A. Kelly
Prolactin (PRL) and Its Receptor: Actions, Signal Transduction Pathways and Phenotypes Observed in PRL Receptor Knockout Mice
Endocr. Rev., June 1, 1998; 19(3): 225 - 268.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
W.-P. Chang, Y. Ye, and C. V. Clevenger
Stoichiometric Structure-Function Analysis of the Prolactin Receptor Signaling Domain by Receptor Chimeras
Mol. Cell. Biol., February 1, 1998; 18(2): 896 - 905.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
G. Luo and L.-y. Yu-Lee
Transcriptional Inhibition by Stat5. DIFFERENTIAL ACTIVITIES AT GROWTH-RELATED VERSUS DIFFERENTIATION-SPECIFIC PROMOTERS
J. Biol. Chem., October 24, 1997; 272(43): 26841 - 26849.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. J. Berlanga, J. P. Garcia-Ruiz, M. Perrot-Applanat, P. A. Kelly, and M. Edery
The Short Form of The Prolactin (PRL) Receptor Silences PRL Induction of the {beta}-Casein Gene Promoter
Mol. Endocrinol., September 1, 1997; 11(10): 1449 - 1457.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
E. A. Burton, S. Hunter, S. C. Wu, and S. M. Anderson
Binding of src-like Kinases to the beta -Subunit of the Interleukin-3 Receptor
J. Biol. Chem., June 27, 1997; 272(26): 16189 - 16195.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. L. Johnson, S. Fenton, and L. G. Sheffield
Prolactin Inhibits Epidermal Growth Factor-induced Ras-MAPK Signaling in Mammary Epithelial Cells
J. Biol. Chem., August 30, 1996; 271(35): 21574 - 21578.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. R. Duan, D. I.H. Linzer, and G. Gibori
Cloning and Characterization of an Ovarian-specific Protein That Associates with the Short Form of the Prolactin Receptor
J. Biol. Chem., June 28, 1996; 271(26): 15602 - 15607.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Blake, A. R. Buckley, M. Zhang, D. J. Buckley, and K. P. Lavoi
A Novel Heat Shock Response in Prolactin-dependent Nb2 Node Lymphoma Cells
J. Biol. Chem., December 8, 1995; 270(49): 29614 - 29620.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. B. Prevarskaya, R. N. Skryma, P. Vacher, N. Daniel, J. Djiane, and B. Dufy
Role of Tyrosine Phosphorylation in Potassium Channel Activation
J. Biol. Chem., October 13, 1995; 270(41): 24292 - 24299.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. V. Clevenger, W. Ngo, D. L. Sokol, S. M. Luger, and , A. M. Gewirtz
Vav Is Necessary for Prolactin-stimulated Proliferation and Is Translocated into the Nucleus of a T-cell Line
J. Biol. Chem., June 2, 1995; 270(22): 13246 - 13253.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. W. Tanner, W. Chen, R. L. Young, G. D. Longmore, and A. S. Shaw
The Conserved Box 1 Motif of Cytokine Receptors Is Required for Association with JAK Kinases
J. Biol. Chem., March 24, 1995; 270(12): 6523 - 6530.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Cheng, I. Zhizhin, R. L. Perlman, and D. Mangoura
Prolactin-induced Cell Proliferation in PC12 Cells Depends on JNK but Not ERK Activation
J. Biol. Chem., July 21, 2000; 275(30): 23326 - 23332.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ali and S. Ali
Recruitment of the Protein-tyrosine Phosphatase SHP-2 to the C-terminal Tyrosine of the Prolactin Receptor and to the Adaptor Protein Gab2
J. Biol. Chem., December 8, 2000; 275(50): 39073 - 39080.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1994 by The Endocrine Society