Molecular Endocrinology, Vol 9, 1214-1222, Copyright © 1995 by Endocrine Society

ARTICLES

The mouse 3 beta-hydroxysteroid dehydrogenase multigene family includes two functionally distinct groups of proteins

IG Abbaszade, TR Clarke, CH Park and AH Payne
Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI 48109-0278, USA.

The enzyme 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) plays an essential role in the biosynthesis of all steroid hormones. We previously reported the isolation, characterization, and tissue- specific expression of four distinct but highly homologous 3 beta HSD cDNAs (forms I, II, III, and IV). Enzymatic characterization of three of these isoforms demonstrated that mouse 3 beta HSD I and III function as dehydrogenase/isomerases, but 3 beta HSD IV functions exclusively as a 3-ketosteroid reductase. We now report the isolation and characterization of an additional distinct mouse 3 beta HSD cDNA, 3 beta HSD V, which is expressed in the liver of male mice beginning in late puberty. Similar to 3 beta HSD IV, 3 beta HSD V functions exclusively as a 3-ketosteroid reductase converting an active androgen, dihydrotestosterone (DHT), into an inactive androgen, 5 alpha- androstane-3 beta,17 beta-diol. Expressed 3 beta HSD V, however, exhibits a considerably lower apparent Michaelis-Menten constant (Km) value for DHT than 3 beta HSD IV (0.47 microM vs. 2.2 microM, respectively). The complete predicted amino acid sequence of 3 beta HSD II is also reported. The predicted amino acid sequence of mouse 3 beta HSD V reveals that this new form is more closely related to the 3- ketosteroid reductases, mouse 3 beta HSD IV and rat III (93 and 84% identity, respectively), than to the other rodent isoforms that share less than 75% identity.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				S. Bhusari, L. B. Hearne, D. E. Spiers, W. R. Lamberson, and E. Antoniou Effect of fescue toxicosis on hepatic gene expression in mice J Anim Sci, June 1, 2006; 84(6): 1600 - 1612. [Abstract] [Full Text] [PDF]

				J. Simard, M.-L. Ricketts, S. Gingras, P. Soucy, F. A. Feltus, and M. H. Melner Molecular Biology of the 3{beta}-Hydroxysteroid Dehydrogenase/{Delta}5-{Delta}4 Isomerase Gene Family Endocr. Rev., June 1, 2005; 26(4): 525 - 582. [Abstract] [Full Text] [PDF]

				P. R. Kiela, A. J. Midura, N. Kuscuoglu, S. D. Jolad, A. M. Solyom, D. G. Besselsen, B. N. Timmermann, and F. K. Ghishan Effects of Boswellia serrata in mouse models of chemically induced colitis Am J Physiol Gastrointest Liver Physiol, April 1, 2005; 288(4): G798 - G808. [Abstract] [Full Text] [PDF]

				A. H. Payne and D. B. Hales Overview of Steroidogenic Enzymes in the Pathway from Cholesterol to Active Steroid Hormones Endocr. Rev., December 1, 2004; 25(6): 947 - 970. [Abstract] [Full Text] [PDF]

				S. Eimerl and J. Orly Regulation of Steroidogenic Genes by Insulin-Like Growth Factor-1 and Follicle-Stimulating Hormone: Differential Responses of Cytochrome P450 Side-Chain Cleavage, Steroidogenic Acute Regulatory Protein, and 3{beta}-Hydroxysteroid Dehydrogenase/Isomerase in Rat Granulosa Cells Biol Reprod, September 1, 2002; 67(3): 900 - 910. [Abstract] [Full Text] [PDF]

				J. C. Crabbe Provisional Mapping of Quantitative Trait Loci for Chronic Ethanol Withdrawal Severity in BXD Recombinant Inbred Mice J. Pharmacol. Exp. Ther., July 1, 1998; 286(1): 263 - 271. [Abstract] [Full Text]

				P. E. Cohen, M. P. Hardy, and J. W. Pollard Colony-Stimulating Factor-1 Plays a Major Role in the Development of Reproductive Function in Male Mice Mol. Endocrinol., October 1, 1997; 11(11): 1636 - 1650. [Abstract] [Full Text]

				I. G. Abbaszade, J. Arensburg, C.-H. J. Park, J. Z. Kasa-Vubu, J. Orly, and A. H. Payne Isolation of a New Mouse 3{beta}-Hydroxysteroid Dehydrogenase Isoform, 3{beta}-HSD VI, Expressed During Early Pregnancy Endocrinology, April 1, 1997; 138(4): 1392 - 1399. [Abstract] [Full Text] [PDF]