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Fig. 5. Dominant Negative Activity by DNA-Binding Mutant of
46-408 ( 46-408 C562G) on GR-Mediated Transcription
CV-1 cells were transfected with 0.1 µg expression vector encoding GR
and 0.3 µg WT AR, 46-408, or 46-408 C562G, 1.7 µg
MMTV-luciferase or TAT-CAT reporter, and 1.0 µg RSV-ß galactosidase
control vector. In some samples, pSG vector was added so that each
sample contained the same amount of DNA. Cells were treated with
106 M dexamethasone and/or testosterone as
indicated for 48 h and harvested, and cell lysates were prepared
and analyzed for luciferase activity as in Fig. 1 and CAT activity in
Materials and Methods. Data are expressed as means
± SD (n = 3). A, MMTV-luciferase reporter study. B,
TAT-CAT reporter study.
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