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Division of Endocrinology, Long Beach Veterans Affairs Medical Center, Long Beach, California 90822; and Departments of Medicine and Pharmacology, University of California, Irvine, California 92717
Address all correspondence and requests for reprints to: Ellis R. Levin, M.D., Medical Service (111-I), Long Beach Veterans Affairs Medical Center/University of California-Irvine, 5901 East 7th Street, Long Beach, California 90822. E-mail: ellis.levin{at}med.va.gov.
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONSIGNALING FROM EGFRs TO...SIGNALING FROM ER THROUGH...PERSPECTIVEREFERENCES |
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resides in or associates with the plasma membrane as a cytoplasmic protein. These ERs utilize the membrane EGFR to rapidly signal through various kinase cascades that influence both transcriptional and nontranscriptional actions of estrogen in breast cancer cells. This is congruent with a general theme of receptor signaling, where membrane G protein-coupled receptors activate tyrosine kinase growth factor receptors (EGFR, IGF-I receptor) that subsequently signal to MAPKs and other pathways. Overall, the bidirectional cross-talk between EGFR and cellular pools of ER contributes to reproductive organ physiology and pathophysiology. |
INTRODUCTION |
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TOPABSTRACTINTRODUCTIONSIGNALING FROM EGFRs TO...SIGNALING FROM ER THROUGH...PERSPECTIVEREFERENCES |
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Kinases also translocate to the nucleus, where they phosphorylate/activate and transcribe transcription factors that induce a variety of immediate early and late-arising genes. This important event underlies many of the biological effects of growth factor signaling. In fact, when nuclear localization of the ERK member of the MAPK family is prevented, cell proliferation often ceases (9). Important nuclear targets of ERK that are relevant to cell division include the transactivation of the cyclin D1 gene and the protooncogenes c-fos and c-myc (10, 11, 12, 13). Therefore, the ability to signal from the membrane to both cytoplasmic and nuclear events is an essential feature of growth factor receptor function.
Steroid hormones have traditionally been conceived to act through the ligation of nuclear receptors (14). For estrogen, binding to estrogen receptor (ER) or ERß results in an active complex in the nucleus that binds DNA directly at estrogen response elements within the promoters of target genes. Alternatively, estradiol (E2)/ER promotes transcription factor binding to DNA (15). Liganded ER forms complexes with coregulator proteins (16), and constituents of the basal transcription machinery complex, leading to the modulation of RNA polymerase II activity, histone-induced chromatin unwinding, and transcription. However, it has become increasingly clear that estrogen (and other steroid hormones) also rapidly activates signaling in seconds to minutes, and this cannot be explained by any known function of nuclear receptors (17). Furthermore, ERs that lack a nuclear localization sequence (18) or truncated ERs that are targeted to the plasma membrane are fully capable of activating kinases and subsequent cell proliferation or survival (19, 20). A small pool of endogenous ERs that localize to the plasma membrane in various target cells can act similarly to classic growth factor receptors imbedded in the membrane. These ERs have been localized to caveolae raft domains isolated from the plasma membrane of target cells such as endothelial cells (19, 21). It is still unclear, however, whether these sex steroid receptors are integral membrane proteins and/or tether as cytoplasmic proteins to the cytoplasmic face of caveolae through binding to caveolin-1.
An important principle in the signaling field is that growth factor receptors cross-talk to each other. This includes heterodimerization between receptor family members, exemplified by the four members of the epidermal growth factor (EGF) receptor (EGFR) family (22). Additionally, signaling from one receptor activates cytoplasmic nonreceptor kinases (e.g. -Src) that positively or negatively modulate the activity of adjacent receptors (23). In this respect, EGFRs expressed on a population of cells may spread signal transduction enacted by a variety of unrelated growth factor receptors on adjacent cells (24).
Emerging data suggest cross-talk may exist between plasma membrane steroid receptors. Progesterone can stimulate ERK signaling via the utilization of ER (25), and estrogen or androgen can promiscuously stimulate signaling to ERK (and cell survival) through either sex steroid receptor (20). Furthermore, both membrane growth factor and steroid receptors interweave their actions with those of nuclear steroid receptors, thereby impacting cell biology. An example is that nuclear receptors transcribe genes, the protein products of which are acutely altered in function by phosphorylation, resulting from membrane receptor signaling.
In this overview, I will describe the current state of cross-talk between ERs and EGFRs. Work in this area has established a requirement of nuclear ER for some EGFR [and perhaps IGF-I receptor (IGF-IR)] actions. Recent findings suggest the important role of EGFR (or similar receptors) for estrogen signaling from the membrane in breast cancer. Bidirectional signaling between these essential cellular factors augments the actions of the individual steroid and growth protein.
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SIGNALING FROM EGFRs TO NUCLEAR ERs |
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TOPABSTRACTINTRODUCTIONSIGNALING FROM EGFRs TO...SIGNALING FROM ER THROUGH...PERSPECTIVEREFERENCES |
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A novel model of ER and EGFR interaction is derived from the observation that EGFR signaling depends upon an ER-mediated function but in an estrogen-independent fashion (32). Studies from Ignar-Trowbridge et al. (33) showed that EGF induction of DNA and lipid synthesis in the uterus could be prevented by ICI 164,384, an ER antagonist. More recent studies suggest that the effects of ER antagonists could be mediated through recruiting corepressors, thereby inhibiting growth factor-induced ER transcriptional effects (34). Continued work from the laboratories of DiAugustine and Korach (35) showed that EGF-induced DNA synthesis and transcription were absent in uteri from ER KO (ERKO) mice. These results clearly show dependency on ER for EGF action in reproductive organs.
How does EGFR utilize ER for biological actions? Insight resulted from the observations that several peptide growth factor receptors signal to the phosphorylation and activation of the nuclear ER (36, 37). This includes EGF and was originally attributed to the ability of growth factor receptor-activated MAPK (ERK) to phosphorylate serine 118 in the A/B domain of the nuclear ER. Serine 118 phosphorylation results in an increased ER-related transactivation of genes that are up-regulated by EGFR. Work by Ignar-Trowbridge et al. (38) showed that EGFR ligation induces the transcriptional up-regulation of an estrogen response element reporter construct, in ER-dependent fashion. This group also demonstrated that EGFR-to-ER cross-talk requires the A/B domain of ER (39). Subsequent studies implicated several kinases that phosphorylate additional residues within ER, resulting in the increased transcriptional activity of the nuclear receptor (40, 41, 42, 43, 44). Thus, impact of the growth factor receptor-ER interaction depends upon the signaling milieu within a particular cell that differentially phosphorylates numerous residues in the nuclear ER.
Another mechanism through which EGFR-induced signaling modulates ER transcriptional activity is via coregulator protein phosphorylation. As an example, EGF-induced ERK phosphorylates serine 736 of glucocorticoid receptor interacting protein 1. This increases the activity of this nuclear receptor nonspecific coactivator protein (45). EGF-triggered Src and Jnk activation may have a similar function for the cAMP response element-binding protein (46). Other coactivator proteins that are important and specific to ER function could be similarly activated or recruited through signaling-induced posttranslational modifications. Interestingly, growth factor receptors signal to cyclin D1 production, as part of promoting G1/S phase cell cycling (47). Cyclin D1 activates ER transcriptional function (48) and interacts with the coactivator proteins, steroid receptor coactivator 1 and cAMP response element-binding protein/p300, as an additional mechanism to amplify nuclear ER action (49, 50).
It is conceivable that EGFR signaling also inhibits the activity/function of corepressor proteins on targeted promoters, and that other EGFR family members could also cross-talk to the nuclear ER. In breast cancer and other estrogen target cells, EGFR family members often heterodimerize, and ligands [heparin binding-EGF (HB-EGF), TGF, or EGF] can be somewhat promiscuous in their binding. Such considerations may be relevant to the interactions of the erb2/Neu oncogene and ER in early breast cancer. In this respect, breast tumor formation in mouse mammary tumor virus-erb2/neu mice is delayed on an ERKO background (51). A summary of mechanisms of EGFR signaling through ER is seen in Fig. 1
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In parallel to the interaction between the EGF system and ER, there is abundant evidence indicating cross-talk between the IGF-I system and ER. IGF-I binding activates its receptor, leading to PI3K/AKT activation, increased ER synthesis, and augmented ER transcriptional activity. This probably results from the phosphorylation of several serine residues in the AF-1 region (43) and EGFR accomplishes a similar action. Similarly to EGF, IGF-I activates parameters of uterine cell proliferation in vivo, and this is dependent on ER (54). Interestingly, in both the uterus and in breast cancer models, IGF-I signaling to ERK and PI3K/AKT is unaffected by ER loss or antagonism (54, 55). When ER is reexpressed in breast cancer cells that have lost ER through repetitive culturing, both E2 and IGF-I resume their growth-inducing function (56). E2 stimulates many proteins in the IGF-I system, including IRS proteins, IGF-IR, and IGF-binding proteins (57, 58), and ER binds and phosphorylates the IGF-1R and enhances signaling through the growth factor receptor (59). In breast cancer, IGF-I and E2 cooperate to promote G1/S cell cycle progression (60, 61), and in the uterus of the IGF-I KO mouse, E2-induced growth is absent (62). Thus, there appears to be an important cooperation and cross-talk between these two systems as well.
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SIGNALING FROM ER THROUGH EGFR |
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TOPABSTRACTINTRODUCTIONSIGNALING FROM EGFRs TO...SIGNALING FROM ER THROUGH...PERSPECTIVEREFERENCES |
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and ERß in Chinese hamster ovary cells (71). These latter studies indicated that membrane ER physically associate with and activate various G protein -subunits, including Gs and Gq. G protein activation explains how ER generates cAMP (Gs function) or inositol 1,4,5-triphosphate and calcium (Gq function), as examples. Subsequent work showed that endogenous ER activates Gi, leading to the generation of nitric oxide in endothelial cells (72).
An important finding described by Ullrich and colleagues (73) indicates that several G protein-coupled receptors (GPCRs) signal to ERK via the transactivation of the EGFR. Later studies from other laboratories confirmed and extended these observations to many GPCRs and provided additional details underlying this cross-talk. Identification of the membrane ER as a receptor capable of activating G proteins (71, 72) invoked the possibility that this receptor signaled through cross-talk/activation of the membrane EGFR. Filardo et al. (74, 75) showed that estrogen rapidly acts in breast cancer cells to stimulate the transactivation of EGFR, leading to cAMP and ERK up-regulation. This occurs through a linked path, first described for other GPCRs by Ullrich and colleagues (76). E2 induces mainly unknown proximal signaling to cause the activation of undefined matrix metalloproteinases (MMPs). Increased MMP function leads to the liberation of HB-EGF, which then binds and activates the EGFR. However, Filardo et al. (74) reported that 17ß-E2, 17-E2, or the ER antagonist, ICI 182780, were equivalent in activating EGFR and ERK. EGFR transactivation was proposed to occur independently of any ER and resulted from an undetermined effect of E2 to activate the orphan GPCR, GPR 30 (77). More recent studies from Razandi et al. (78) demonstrated that E2 requires an ER to signal to EGFR in breast cancer and is consistent with most studies that show an ER is necessary for rapid signaling by E2 at the membrane (19, 42, 66, 79, 80, 81). Razandi et al. (78) also found that E2/ER triggers a Gq, Gi, and Gß
-dependent activation of MMP-2 and -9, mediated through Src activation. By antisense studies, MMP-2 and MMP-9 were shown to be necessary for E2-induced HB-EGF cleavage and liberation, the transactivation of EGFR, and downstream signaling to ERK and PI3K in breast cancer cells, and p38 MAPK in endothelial cells. It is possible that GPR30 may complex with and mediate membrane ER cross-talk to EGFR. However, recent studies from Ahola et al. (82) have called this idea into question. These investigators found that antisense inhibition of endogenous GPR-30 had no effect on E2 signaling to cell proliferation in MCF-7 cells. Thus, this definitive approach suggests that GPR30 is not required and supports previous studies that ER 1) directly associates with and activates G proteins, and 2) this leads to downstream signaling (71, 72). The molecules involved in the ER-to-EGFR cross-talk are shown in Fig. 2.
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directly associates with the membrane-tethered p85 subunit of PI3K. E2 rapidly activates this kinase, leading to the generation of nitric oxide, and the rescue of rats from ischemia-reperfusion injury of their muscle. It is known that EGFR and PI3K associate (84), and so it is possible that a multiprotein complex exists between ER/PI3K/EGFR and endothelial nitric oxide synthase molecules, perhaps scaffolded onto caveolin-1 at the membrane (19, 21, 85). Similarly, Migliaccio et al. (79) showed that ER and Src form a complex. The interaction between ER and Src may be mediated by a newly described adapter protein, modulator of nongenomic activity of estrogen receptor (86). Src activation by E2 leads to a kinase cascade resulting in ERK activation and DNA synthesis in cancer cells (79). Interestingly, EGFR and Src associate, and both molecules also form complexes with caveolin-1 (87). Src or EGFR phosphorylates caveolin-1 at the important tyrosine 14, and this leads to the down-regulation of signaling (88). The ability of E2/ER at the membrane to signal to ERK (via the demonstrated EGFR transactivation) has additional importance for cell biology. Song et al. (89) recently demonstrated that ER lacking a nuclear localization signal and targeted to the plasma membrane activates ERK and cell proliferation in Chinese hamster ovary cells. Also, the survival of breast cancer cells that are subjected to radiation or taxol chemotherapy is enhanced by E2, partially through ERK activation (90). In aggressive breast cancer, a truncated MTA1 protein was recently found to be highly expressed (91). This protein sequesters ER away from the nucleus and strongly reduces E2-activated transcription, yet promotes increased ERK signaling and aggressive behavior of the tumor. In neurons subjected to several inducers of apoptosis, E2 protects these cells through ERK activation (92). The actions of E2 mediated by this MAPK occur through both protein phosphorylation (90) and gene transcription (93, 94). Most recently, bone loss in vivo was prevented by a compound (4-estren-3, 17ß-diol) that has no direct transcriptional activities but activates ERK signaling (95). Therefore, it is probable that the cross-talk from membrane ER through EGFR to downstream kinase activation is biologically important.
The precise structural aspects of the membrane ER that are required for G protein activation are unclear at present but appear to mainly reside in the E domain. This conclusion is based upon the observations that targeting the E domain alone to the plasma membrane allows E2 activation of ERK (19) and rescues bone cells from an apoptotic cell death (20). Similarly, sending the E domain to the plasma membrane of ER-negative, breast cancer cells results in E2-induced, Src-dependent matrix metalloproteinase activation, HB-EGF liberation, and EGFR transactivation (78). Thus, the membrane E domain alone can recapitulate the key elements of the pathway from ER to EGFR. These findings are supported by the earlier observation that Src complexes with (and is activated by) E2/ER, and that tyrosine 537 within the E domain is an essential structural component (96). This may be important for specific signaling pathways, however. Bjornstrom and Sjoberg (97) have recently examined the E2 rapid activation of signal transducer and activator of transcription (STAT) transcription factor-induced ß-casein promoter activation. STAT activation requires both ERK and PI3K, induced by E2/ER. These authors report that mutating tyrosine 541 of the mouse ER (equivalent of human ER tyrosine 537) has no effect on E2 induction of the STAT-ß-casein pathway. Also, Song et al. (89) recently showed that the Src homology 2-domain containing adapter protein, Shc, complexes with ER through the AF-1 domain, and suggested that this interaction may underlie E2-induced ERK. However, we recently found that expression of only the membrane-targeted E domain (19), or A/B domain-deleted ER (unpublished observations) 1) fully binds steroid at the membrane, and 2) comparably activates ERK, compared with expressed wild-type ER. Thus, current data support a unique and complete role for the E domain in effecting signal transduction initiated at the membrane.
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PERSPECTIVE |
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TOPABSTRACTINTRODUCTIONSIGNALING FROM EGFRs TO...SIGNALING FROM ER THROUGH...PERSPECTIVEREFERENCES |
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The interactions of ER and EGFR impact both the transcriptional and nontranscriptional effects of steroid hormones and protein growth factors, but these are not mutually exclusive actions. Membrane E2/ER activates PI3K signaling via EGFR (78). As shown by DNA microarray, PI3K activation by E2 leads to the up-regulation of 250 genes after just 40 min of exposure of endothelial cells to sex steroid (100). Thus, ER-EGFR cross-talk at the membrane enacts multiple signaling pathways that likely have a profound impact on the transcriptional effects of E2. It is certainly possible that manipulating the specific pathways that result from the bidirectional signaling will yield therapeutic interventions for human disorders that result from excessive growth factor and steroid hormone action.
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ACKNOWLEDGMENTS |
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FOOTNOTES |
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Abbreviations: E2, Estradiol; EGF, epidermal growth factor; EGFR, EGF receptor; ER, estrogen receptor; GPCR, G protein-coupled receptor; HB-EGF, heparin binding-EGF; IGF-IR, IGF-I receptor; KO, knockout; MMP, matrix metalloproteinase; PI3K, phosphatidylinositol 3-kinase; STAT, signal transducer and activator of transcription.
Received for publication November 6, 2002. Accepted for publication December 6, 2002.
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TOPABSTRACTINTRODUCTIONSIGNALING FROM EGFRs TO...SIGNALING FROM ER THROUGH...PERSPECTIVEREFERENCES |
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mediates estrogen effect on MAPK activation. Biochem Biophys Res Commun 294:926–933[CrossRef][Medline]
and endothelial nitric oxide synthase are organized into a functional signaling module in caveolae. Circ Res 87:e44–e52
provides evidence in support of a role for corepressors in regulating cellular responses to agonists and antagonists. Mol Endocrinol 16:1778–1792 at serine 118 by two distinct signal transduction pathways revealed by phosphorylation-specific antisera. Oncogene 21:4921–4931[CrossRef][Medline]
and promotes hyperplasia in mammary epithelium. EMBO J 21:5437–5447[CrossRef][Medline]
delays mammary tumor formation induced by transgenic expression of ErbB2/neu. Cancer Res 62:2798–2805 in insulin-like growth factor-1 (IGF-1)-induced uterine responses and in vivo evidence for IGF-1/estrogen receptor cross talk. J Biol Chem 277:8531–8537 in estrogen receptor -negative MCF-7 cells restores both estrogen and insulin-like growth factor-mediated signaling and growth. Cancer Res 61:5771–5777 rapidly activates the IGF-1 receptor pathway. J Biol Chem 275:18447–18453 detected on the plasma membrane of aldehyde-fixed GH3/B6/F10 rat pituitary tumor cells by enzyme-linked immunocytochemistry. Endocrinology 140:3805–3814 and ERß expressed in CHO cells. Mol Endocrinol 13:307–319(i). J Biol Chem 276:27071–27076-Shc association and Shc pathway activation. Mol Endocrinol 16:116–127NURSA Molecule Pages Link:
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A. Migliaccio, M. Di Domenico, G. Castoria, M. Nanayakkara, M. Lombardi, A. de Falco, A. Bilancio, L. Varricchio, A. Ciociola, and F. Auricchio Steroid Receptor Regulation of Epidermal Growth Factor Signaling through Src in Breast and Prostate Cancer Cells: Steroid Antagonist Action Cancer Res., November 15, 2005; 65(22): 10585 - 10593. [Abstract] [Full Text] [PDF]
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J. L. Boerner, M. A. Gibson, E. M. Fox, E. D. Posner, S. J. Parsons, C. M. Silva, and M. A. Shupnik Estrogen Negatively Regulates Epidermal Growth Factor (EGF)-Mediated Signal Transducer and Activator of Transcription 5 Signaling in Human EGF Family Receptor-Overexpressing Breast Cancer Cells Mol. Endocrinol., November 1, 2005; 19(11): 2660 - 2670. [Abstract] [Full Text] [PDF]
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 F. F. Zheng, R.-C. Wu, C. L. Smith, and B. W. O'Malley Rapid Estrogen-Induced Phosphorylation of the SRC-3 Coactivator Occurs in an Extranuclear Complex Containing Estrogen Receptor Mol. Cell. Biol., September 15, 2005; 25(18): 8273 - 8284. [Abstract] [Full Text] [PDF]
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R. K. Vadlamudi, B. Manavathi, S. Balasenthil, S. S. Nair, Z. Yang, A. A. Sahin, and R. Kumar Functional Implications of Altered Subcellular Localization of PELP1 in Breast Cancer Cells Cancer Res., September 1, 2005; 65(17): 7724 - 7732. [Abstract] [Full Text] [PDF]
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T. Takahashi, M. Ohmichi, J. Kawagoe, C. Ohshima, M. Doshida, T. Ohta, M. Saitoh, A. Mori-Abe, B. Du, H. Igarashi, et al. Growth Factors Change Nuclear Distribution of Estrogen Receptor-{alpha} via Mitogen-Activated Protein Kinase or Phosphatidylinositol 3-Kinase Cascade in a Human Breast Cancer Cell Line Endocrinology, September 1, 2005; 146(9): 4082 - 4089. [Abstract] [Full Text] [PDF]
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J. H. Gutzman, S. E. Nikolai, D. E. Rugowski, J. J. Watters, and L. A. Schuler Prolactin and Estrogen Enhance the Activity of Activating Protein 1 in Breast Cancer Cells: Role of Extracellularly Regulated Kinase 1/2-Mediated Signals to c-fos Mol. Endocrinol., July 1, 2005; 19(7): 1765 - 1778. [Abstract] [Full Text] [PDF]
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C. Grossmann, A. Benesic, A. W. Krug, R. Freudinger, S. Mildenberger, B. Gassner, and M. Gekle Human Mineralocorticoid Receptor Expression Renders Cells Responsive for Nongenotropic Aldosterone Actions Mol. Endocrinol., July 1, 2005; 19(7): 1697 - 1710. [Abstract] [Full Text] [PDF]
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 J. D. Patel Lung Cancer in Women J. Clin. Oncol., May 10, 2005; 23(14): 3212 - 3218. [Abstract] [Full Text] [PDF]
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 N. Kanda and S. Watanabe 17{beta}-Estradiol enhances heparin-binding epidermal growth factor-like growth factor production in human keratinocytes Am J Physiol Cell Physiol, April 1, 2005; 288(4): C813 - C823. [Abstract] [Full Text] [PDF]
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C. K. Osborne and R. Schiff Estrogen-Receptor Biology: Continuing Progress and Therapeutic Implications J. Clin. Oncol., March 10, 2005; 23(8): 1616 - 1622. [Full Text] [PDF]
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 S. Banerjee, K. Sengupta, N. K. Saxena, K. Dhar, and S. K. Banerjee Epidermal Growth Factor Induces WISP-2/CCN5 Expression in Estrogen Receptor-{alpha}-Positive Breast Tumor Cells through Multiple Molecular Cross-talks Mol. Cancer Res., March 1, 2005; 3(3): 151 - 162. [Abstract] [Full Text] [PDF]
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L. P. Stabile, J. S. Lyker, C. T. Gubish, W. Zhang, J. R. Grandis, and J. M. Siegfried Combined Targeting of the Estrogen Receptor and the Epidermal Growth Factor Receptor in Non-Small Cell Lung Cancer Shows Enhanced Antiproliferative Effects Cancer Res., February 15, 2005; 65(4): 1459 - 1470. [Abstract] [Full Text] [PDF]
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P. A. Hershberger, A. C. Vasquez, B. Kanterewicz, S. Land, J. M. Siegfried, and M. Nichols Regulation of Endogenous Gene Expression in Human Non-Small Cell Lung Cancer Cells by Estrogen Receptor Ligands Cancer Res., February 15, 2005; 65(4): 1598 - 1605. [Abstract] [Full Text] [PDF]
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 Q. Lu, D. C. Pallas, H. K. Surks, W. E. Baur, M. E. Mendelsohn, and R. H. Karas Striatin assembles a membrane signaling complex necessary for rapid, nongenomic activation of endothelial NO synthase by estrogen receptor {alpha} PNAS, December 7, 2004; 101(49): 17126 - 17131. [Abstract] [Full Text] [PDF]
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M. Razandi, A. Pedram, I. Merchenthaler, G. L. Greene, and E. R. Levin Plasma Membrane Estrogen Receptors Exist and Functions as Dimers Mol. Endocrinol., December 1, 2004; 18(12): 2854 - 2865. [Abstract] [Full Text] [PDF]
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M. Razandi, A. Pedram, E. M. Rosen, and E. R. Levin BRCA1 Inhibits Membrane Estrogen and Growth Factor Receptor Signaling to Cell Proliferation in Breast Cancer Mol. Cell. Biol., July 1, 2004; 24(13): 5900 - 5913. [Abstract] [Full Text] [PDF]
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 R. I. Fernando and J. Wimalasena Estradiol Abrogates Apoptosis in Breast Cancer Cells through Inactivation of BAD: Ras-dependent Nongenomic Pathways Requiring Signaling through ERK and Akt Mol. Biol. Cell, July 1, 2004; 15(7): 3266 - 3284. [Abstract] [Full Text] [PDF]
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K. Sengupta, S. Banerjee, N. K. Saxena, and S. K. Banerjee Thombospondin-1 Disrupts Estrogen-Induced Endothelial Cell Proliferation and Migration and Its Expression Is Suppressed by Estradiol Mol. Cancer Res., March 1, 2004; 2(3): 150 - 158. [Abstract] [Full Text] [PDF]
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D.-b. Chen, I. M. Bird, J. Zheng, and R. R. Magness Membrane Estrogen Receptor-Dependent Extracellular Signal-Regulated Kinase Pathway Mediates Acute Activation of Endothelial Nitric Oxide Synthase by Estrogen in Uterine Artery Endothelial Cells Endocrinology, January 1, 2004; 145(1): 113 - 125. [Abstract] [Full Text] [PDF]
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J. S. Foster, R. I. Fernando, N. Ishida, K. I. Nakayama, and J. Wimalasena Estrogens Down-regulate p27Kip1 in Breast Cancer Cells through Skp2 and through Nuclear Export Mediated by the ERK Pathway J. Biol. Chem., October 17, 2003; 278(42): 41355 - 41366. [Abstract] [Full Text] [PDF]
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