Human Loss-of-Function Gonadotropin-Releasing Hormone Receptor Mutants Retain Wild-Type Receptors in the Endoplasmic Reticulum: Molecular Basis of the Dominant-Negative Effect
Mol Endocrinol Brothers et al.
18: 1787
Online Supplemental Material
Online Supplemental Material:
Movies of 3D reconstructions from COS7 cells in figure 4C and 4D co-transfected with the GFP-tagged WT hGnRHR and hGnRHR(E90K) cDNA, treated with vehicle and IN3, respectively are shown (Figs. S1 and S2). Cells were digitally transected to show intracellular contents. Green represents the GFP, blue represents the ER and turquoise represents areas of overlap.
Files in this Data Supplement:
- Figure S1
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Figure S1. Movie of 3D reconstruction from COS7 cell in figure 4C. Five ng of GFP-tagged WT hGnRHR was coexpressed with 95 ng of hGnRHR(E90K) and treated with DMSO (vehicle). The ER was stained blue using ER-tracker™ Dye. Confocal sections were taken and processed into 3D reconstructions using Volocity 1.4.3 (Improvision Inc., Lexington, MA).
- Figure S2
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Figure S2. Movie of 3D reconstruction from COS7 cell in figure 4D. Five ng of GFP-tagged WT hGnRHR was coexpressed with 95 ng of hGnRHR(E90K) and treated with IN3 (vehicle). The ER was stained blue using ER-tracker™ Dye. Confocal sections were taken and processed into 3D reconstructions using Volocity 1.4.3 (Improvision Inc., Lexington, MA).
