Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Amhr2^cre/+SmoM2 mice have prolonged estrous cycles, characterized by extended periods of diestrus. Vaginal smears were evaluated for the stage of the estrous cycle in four Amhr2^+/+SmoM2 mice and four Amhr2^cre/+SmoM2 mice for 3 complete cycles or 20 days. The left panel shows cycle length, and the right panel shows the percent of days mice exhibited smears characteristic of each stage of the cycle. Data are mean ± SEM. *, P < 0.05 vs Amhr2^+/+SmoM2 mice.
• Supplemental Figure 2 - Staining for the smooth muscle marker, SMA, and the endothelial cell marker, VWB, in ovaries of Amhr2^+/+SmoM2 (top panels) and Amhr2^cre/+SmoM2 mice (bottom panels). Sections were co-stained for SMA (red) and VWB (green) and for nuclei (DAPI; blue) and individual and merged images are shown. Sections are from ovaries isolated 48 h after injection of eCG to immature mice.
• Supplemental Figure 3 - Granulosa cells from preovulatory follicles of eCG-primed Amhr2^+/+SmoM2 and Amhr2^cre/+SmoM2 mice begin to exit the cell cycle in a similar manner after injection of hCG. Cell cycle stage was determined by flow cytometric analysis of propidium iodide binding to cellular DNA. Data are mean ± SEM. n = 3-8 granulosa cell preparations from individual animals. Within each cell cycle stage, bars without common superscripts are significantly different (P < 0.05).
• Supplemental Table 1 - Supplemental Table 1