Supplemental Data

Supplemental Data

Files in this Data Supplement:

• Supplementary Figure 1 - Differentially expressed genes during pregnancy day 14.5 (FC 1.5 and FDR 20%)
• Supplementary Figure 2 - Separation of GFP+ and GFP- populations from Mip-GFP mice. (A) SSC (side scatter) is proportional to cell complexity and depends on the number of organelles inside the cell. FSC (forward scatter) correlates with cell size. GFP 530/30-A measures strength of an individual cell’s GFP intensity. (A-D) Freshly isolated islets from CD1+ female mouse (no GFP expression) were used to calibrate the cell sorter before GFP sorting began. (E) Plotting both SSC and FSC shows all cells collected as shown by the highlighted gate. (F) Only singlet or non-aggregated cells were used for efficient cell sorting. (G-H) Two distinct populations of cells were collected separately for further analysis.