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A Novel Estrogen Receptor -Associated Protein Alters Receptor-Deoxyribonucleic Acid Interactions and Represses Receptor-Mediated Transcription

Department of Molecular and Integrative Physiology (M.A.L., R.E.D., C.D.C., A.M.N.), University of Illinois, Urbana, Illinois 61801; Department of Biological Sciences (N.M.), University of California, Irvine, California 92697; and Department of Cell Biology (J.R.Y.), The Scripps Research Institute, La Jolla, California 92037

Address all correspondence and requests for reprints to: Ann M. Nardulli, Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, 524 Burrill Hall, 407 South Goodwin Avenue, Urbana, Illinois 61801. E-mail: anardull{at}life.uiuc.edu.

Estrogen receptor (ER) serves as a ligand-activated transcription factor, turning on transcription of estrogen-responsive genes in target cells. Numerous regulatory proteins interact with the receptor to influence ER-mediated transactivation. In this study, we have identified pp32, which interacts with the DNA binding domain of ER when the receptor is free, but not when it is bound to an estrogen response element. Coimmunoprecipitation experiments demonstrate that endogenously expressed pp32 and ER from MCF-7 breast cancer cells interact. Although pp32 substantially enhances the association of the receptor with estrogen response element-containing DNA, overexpression of pp32 in MCF-7 cells decreases transcription of an estrogen-responsive reporter plasmid. pp32 Represses p300-mediated acetylation of ER and histones in vitro and inhibits acetylation of ER in vivo. pp32 Also binds to other nuclear receptors and inhibits thyroid hormone receptor ß-mediated transcription. Taken together, our studies provide evidence that pp32 plays a role in regulating transcription of estrogen-responsive genes by modulating acetylation of histones and ER and also influences transcription of other hormone-responsive genes as well.

NURSA Molecule Pages Link:

Nuclear Receptors:   TRβ  |  PPARγ  |  RXRα  |  ERα  |  PR

Coregulators:   pp32  |  p300

Ligands:   17β-Estradiol

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