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Identification of Estrogen-Responsive Genes by Complementary Deoxyribonucleic Acid Microarray and Characterization of a Novel Early Estrogen-Induced Gene: EEIG1

Department of Chemical Engineering and Applied Chemistry (D.-Y.W., E.A.E.) and Banting and Best Department of Medical Research (D.-Y.W.), University of Toronto, Toronto, Ontario M5S 3E5; Department of Physical and Environmental Sciences (R.F.), University of Toronto at Scarborough, Scarborough, Ontario M1C 1A4; and Department of Chemistry, Biology and Chemical Engineering (S.N.L.), Ryerson University, Toronto, Ontario M5B 2K3 Canada

Address all correspondence and requests for reprints to: Elizabeth A. Edwards, Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario M5S 3E5, Canada. E-mail: edwards{at}chem-eng.utoronto.ca.

Estrogen receptors (ERs) are nuclear transcription factors that regulate gene expression in response to estrogen and estrogen-like compounds. Identification of estrogen-regulated genes in target cells is an essential step toward understanding the molecular mechanisms of estrogen action. Using cDNA microarray examinations, 19 genes were identified as induced by 17ß-estradiol in MCF-7 cells, 10 of which have been reported previously to be estrogen responsive or to be linked with ER status. Five known estrogen-regulated genes, E2IG4, IGFBP4, SLC2A1, XBP1 and B4GALT1, and AFG3L1, responded quickly to estrogen treatment. A novel estrogen-responsive gene was identified and named EEIG1for early estrogen-induced gene 1. EEIG1 was clearly induced by 17ß-estradiol within 2 h of treatment, and was widely responsive to a group of estrogenic compounds including natural and synthetic estrogens and estrogenic environmental compounds. EEIG1 was expressed in ER-positive but not in ER-negative breast cancer cell lines. EEIG1 expression was repressed by antiestrogens 4-OH-tamoxifen and ICI 182,780 but not by protein synthesis inhibitors cycloheximide and puromycin. These results provide evidence that some estrogenic compounds differentially enhance the transcription of estrogen-regulated genes and suggest a role for EEIG1 in estrogen action.

NURSA Molecule Pages Link:

Nuclear Receptors:   ERα

Ligands:   17β-Estradiol  |  Dihydrotestosterone  |  Diethylstilbestrol  |  4-Hydroxytamoxifen

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