| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on January 9, 2002
Accepted on July 16, 2002
1 Departments of Physiologyand Medicine, University of Toronto and Division of Cellular and Molecular Biology, Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada M5S 1A8
* To whom correspondence should be addressed. E-mail: d.belsham{at}utoronto.ca.
Steroid hormones induce rapid membrane receptor-mediated effects that appear to be separate from long term genomic events. The membrane receptor-mediated effects of androgens on GT1
7 GnRH-secreting neurons were examined. We observed androgen binding activity with a cell impermeable BSA-conjugated testosterone (T-3-BSA) and are able to detect a 110 kDa protein recognized by the androgen receptor (AR) monoclonal MA1150 antibody in the plasma membrane fraction of the GT17 cells by Western analysis. Further, a transfected green fluorescent protein-tagged AR translocates and colocalizes to the plasma membrane of the GT17 neuron. Treatment with 10 nM 5
-dihydrotestosterone (DHT) inhibits forskolin-stimulated accumulation of adenosine 3',5'-cyclic monophosphate (cAMP), through a pertussis toxin-sensitive G protein, but has no effect on basal cAMP levels. The inhibition of forskolin-stimulated cAMP accumulation by DHT was blocked by hydroxyflutamide, a specific inhibitor of the nuclear AR. DHT, T, and T-3-BSA, all caused significant elevations in intracellular calcium concentrations ([Ca2+]i). T-3-BSA stimulates GnRH secretion two-fold in the GT17 neuron, as did DHT or T. Interestingly GnRH mRNA levels were down-regulated by DHT and T as has been reported, but not by treatment with T-3-BSA or T-17-BSA. These studies indicate that androgen can differentially regulate GnRH secretion and gene expression through specific membrane-mediated or nuclear mechanisms.
7 neurons •
membrane androgen receptor •
secretion •
transcription
NURSA Molecule Pages Link:
This article has been cited by other articles:
 |
|
 |
|
  P. Kumar, Q. Wu, K. L. Chambliss, I. S. Yuhanna, S. M. Mumby, C. Mineo, G. G. Tall, and P. W. Shaul Direct Interactions with G{alpha}i and G{beta}{gamma} Mediate Nongenomic Signaling by Estrogen Receptor {alpha} Mol. Endocrinol., June 1, 2007; 21(6): 1370 - 1380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. El-Etr, Y. Akwa, E.-E. Baulieu, and M. Schumacher The Neuroactive Steroid Pregnenolone Sulfate Stimulates the Release of Gonadotropin-Releasing Hormone from GT1-7 Hypothalamic Neurons, through N-Methyl-D-Aspartate Receptors Endocrinology, June 1, 2006; 147(6): 2737 - 2743. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Pielecka and S. M. Moenter Effect of Steroid Milieu on Gonadotropin-Releasing Hormone-1 Neuron Firing Pattern and Luteinizing Hormone Levels in Male Mice Biol Reprod, May 1, 2006; 74(5): 931 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Maier, D. Runzler, J. Schindelar, G. Grabner, W. Waldhausl, G. Kohler, and A. Luger G-protein-coupled glucocorticoid receptors on the pituitary cell membrane J. Cell Sci., August 1, 2005; 118(15): 3353 - 3361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Iranmanesh and J. D. Veldhuis Combined Inhibition of Types I and II 5 {alpha}-Reductase Selectively Augments the Basal (Nonpulsatile) Mode of Testosterone Secretion in Young Men J. Clin. Endocrinol. Metab., July 1, 2005; 90(7): 4232 - 4237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Cheng and P. C. K. Leung Molecular Biology of Gonadotropin-Releasing Hormone (GnRH)-I, GnRH-II, and Their Receptors in Humans Endocr. Rev., April 1, 2005; 26(2): 283 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Fix, C. Jordan, P. Cano, and W. H. Walker Testosterone activates mitogen-activated protein kinase and the cAMP response element binding protein transcription factor in Sertoli cells PNAS, July 27, 2004; 101(30): 10919 - 10924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Sullivan and S. M. Moenter Prenatal androgens alter GABAergic drive to gonadotropin-releasing hormone neurons: Implications for a common fertility disorder PNAS, May 4, 2004; 101(18): 7129 - 7134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Watson and B. Gametchu Proteins of Multiple Classes May Participate in Nongenomic Steroid Actions Experimental Biology and Medicine, December 1, 2003; 228(11): 1272 - 1281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Gros, T. Afroze, X.-M. You, G. Kabir, R. Van Wert, W. Kalair, A. E. Hoque, I. N. Mungrue, and M. Husain Plasma Membrane Calcium ATPase Overexpression in Arterial Smooth Muscle Increases Vasomotor Responsiveness and Blood Pressure Circ. Res., October 3, 2003; 93(7): 614 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Liao, J. B. Thrasher, J. Pelling, J. Holzbeierlein, Q.-X. A. Sang, and B. Li Androgen Stimulates Matrix Metalloproteinase-2 Expression in Human Prostate Cancer Endocrinology, May 1, 2003; 144(5): 1656 - 1663. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
