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Submitted on March 20, 2002
Accepted on August 22, 2002
1 Division of Endocrinology, Department of Medicine, and Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, McGill University, Montréal, QC, Canada H3T 1E2, Calcium Research Laboratory, Department of Medicine, McGill University Health Centre and McGill University, Montréal, QC, Canada H3A 1A1, Faculty of Dentistry, and Department of Anatomy and Cell Biology, McGill University, Montréal, QC, Canada H3A 2B2
* To whom correspondence should be addressed. E-mail: akarapli{at}ldi.jgh.mcgill.ca.
Inactivating mutations and/or deletions of PHEX/Phex are responsible for X-linked hypophosphatemic rickets (XLH) in humans and in the murine homologue, Hyp. The predominant osteoblastic expression of Phex has implicated a primary metabolic osteoblast defect in the pathophysiology of this disorder. By targeting PHEX expression to osteoblasts in the Hyp genetic background, we aimed to correct the corresponding biochemical and morphological abnormalities and obtain information on their pathogenetic mechanism. When transgene Phex expression, driven by a mouse pro-
1(I) collagen gene promoter, was crossed into the Hyp background, it improved the defective mineralization of bone and teeth but failed to correct the hypophosphatemia and altered vitamin D metabolism associated with the disorder. Ex-vivo bone marrow cultures confirmed the amelioration in the Hyp-associated matrix mineralization defect after Phex expression. These findings suggest that while the Hyp bone and teeth abnormalities partially correct after PHEX gene transfer, additional factors and/or sites of PHEX expression are likely critical for the elaboration of the appropriate molecular signals that alter renal phosphate handling and vitamin D metabolism in this disorder.
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