help button home button Endocrine Society Molecular Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
This version published online on May 1, 2003
Molecular Endocrinology, doi:10.1210/me.2002-0286
A more recent version of this article appeared on August 1, 2003
This Article
Right arrow Author Manuscript (PDF)
Right arrow All Versions of this Article:
17/8/1470    most recent
Author Manuscript (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow NURSA Molecule Pages Link
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jacobs, S. B.R.
Right arrow Articles by Mellon, P. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jacobs, S. B.R.
Right arrow Articles by Mellon, P. L.

Submitted on August 19, 2002
Accepted on April 22, 2003

Nuclear Factor-Y and Steroidogenic Factor-1 Physically and Functionally Interact to Contribute to Cell-Specific Expression of the Mouse FSH{beta} Gene

Suzanne B.R. Jacobs1, Djurdjica Coss1, Shauna M. McGillivray1, and Pamela L. Mellon1*

1 Department of Reproductive Medicine, University of California, San Diego, La Jolla, California 92093-0674

* To whom correspondence should be addressed. E-mail: pmellon{at}ucsd.edu.

FSH (FSH) is a heterodimeric glycoprotein hormone secreted from the gonadotrope cell population of the anterior pituitary. Despite its crucial role in mammalian reproduction, very little is known about regulation of the FSH {beta}-subunit gene at the molecular level. In this report, we examine the basis for cell-specific expression of FSH{beta} using the mouse L{beta}T2 and {alpha}T3-1 gonadotrope-derived cell lines. Characterization of the hormonal content of L{beta}T2 and {alpha}T3-1 cells at the protein level classifies these cells as relatively mature and immature gonadotropes, respectively. We studied L{beta}T2 cell-specific expression of FSH{beta} using 398 base-pairs of the mouse FSH{beta} regulatory region linked to a luciferase reporter gene in transient transfection assays. This mouse FSH{beta} promoter can direct reporter gene expression specifically to L{beta}T2 cells when compared with other pituitary and nonpituitary-derived cell lines, including {alpha}T3-1 cells. Furthermore, it is induced by activin and interruption of the autocrine activin loop in L{beta}T2 cells by the addition of follistatin reduces its expression. Truncation analysis indicates that several regions of the promoter are involved in this specificity and that these can be dissociated from activin regulation. We identify binding sites for the orphan nuclear receptor steroidogenic factor-1 (SF-1) and the heterotrimeric transcription factor nuclear factor-Y (NF-Y) and show that these elements functionally interact to regulate FSH{beta} gene expression in an L{beta}T2 cell-specific manner. Moreover, SF-1 and NF-Y are shown to physically interact with each other. This study is the first to demonstrate the presence of basal FSH{beta} protein in L{beta}T2 cells and to identify specific elements within the FSH{beta} promoter that contribute to basal and cell-specific expression of the gene.
Key words: FSH • steroidogenic factor 1 • nuclear factor-Y • pituitary gonadotrope

NURSA Molecule Pages Link:

Nuclear Receptors:   SF-1



This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
N. A. Ciccone, C. T. Lacza, M. Y. Hou, S. J. Gregory, K.-Y. Kam, S. Xu, and U. B. Kaiser
A Composite Element that Binds Basic Helix Loop Helix and Basic Leucine Zipper Transcription Factors Is Important for Gonadotropin-Releasing Hormone Regulation of the Follicle-Stimulating Hormone {beta} Gene
Mol. Endocrinol., August 1, 2008; 22(8): 1908 - 1923.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
X. Zhu, A. S. Gleiberman, and M. G. Rosenfeld
Molecular Physiology of Pituitary Development: Signaling and Transcriptional Networks
Physiol Rev, July 1, 2007; 87(3): 933 - 963.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
W. Zheng, J. Yang, Q. Jiang, Z. He, and L. M Halvorson
Liver receptor homologue-1 regulates gonadotrope function
J. Mol. Endocrinol., February 1, 2007; 38(2): 207 - 219.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
K. B Lee, V. Khivansara, M. M Santos, P. Lamba, T. Yuen, S. C Sealfon, and D. J Bernard
Bone morphogenetic protein 2 and activin A synergistically stimulate follicle-stimulating hormone {beta} subunit transcription
J. Mol. Endocrinol., February 1, 2007; 38(2): 315 - 330.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
V. G. Thackray, S. M. McGillivray, and P. L. Mellon
Androgens, Progestins, and Glucocorticoids Induce Follicle-Stimulating Hormone {beta}-Subunit Gene Expression at the Level of the Gonadotrope
Mol. Endocrinol., September 1, 2006; 20(9): 2062 - 2079.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Xu, Y. L. Zhou, W. Luo, Q.-S. Zhu, D. Levy, O. A. MacDougald, and M. L. Snead
NF-Y and CCAAT/Enhancer-binding Protein {alpha} Synergistically Activate the Mouse Amelogenin Gene
J. Biol. Chem., June 9, 2006; 281(23): 16090 - 16098.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
P. Lamba, M. M Santos, D. P Philips, and D. J Bernard
Acute regulation of murine follicle-stimulating hormone {beta} subunit transcription by activin A
J. Mol. Endocrinol., February 1, 2006; 36(1): 201 - 220.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Rave-Harel, N. L. G. Miller, M. L. Givens, and P. L. Mellon
The Groucho-related Gene Family Regulates the Gonadotropin-releasing Hormone Gene through Interaction with the Homeodomain Proteins MSX1 and OCT1
J. Biol. Chem., September 2, 2005; 280(35): 30975 - 30983.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
K.-Y. Kam, K.-H. Jeong, E. R. Norwitz, E. M. Jorgensen, and U. B. Kaiser
Oct-1 and Nuclear Factor Y Bind to the SURG-1 Element to Direct Basal and Gonadotropin-Releasing Hormone (GnRH)-Stimulated Mouse GnRH Receptor Gene Transcription
Mol. Endocrinol., January 1, 2005; 19(1): 148 - 162.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. E. West, G. E. Parker, J. J. Savage, P. Kiratipranon, K. S. Toomey, L. R. Beach, S. C. Colvin, K. W. Sloop, and S. J. Rhodes
Regulation of the Follicle-Stimulating Hormone {beta} Gene by the LHX3 LIM-Homeodomain Transcription Factor
Endocrinology, November 1, 2004; 145(11): 4866 - 4879.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q.-S. Zhu, B. Qian, and D. Levy
CCAAT/Enhancer-binding Protein {alpha} (C/EBP{alpha}) Activates Transcription of the Human Microsomal Epoxide Hydrolase Gene (EPHX1) through the Interaction with DNA-bound NF-Y
J. Biol. Chem., July 16, 2004; 279(29): 29902 - 29910.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
T. J. Spady, R. Shayya, V. G. Thackray, L. Ehrensberger, J. S. Bailey, and P. L. Mellon
Androgen Regulates Follicle-Stimulating Hormone {beta} Gene Expression in an Activin-Dependent Manner in Immortalized Gonadotropes
Mol. Endocrinol., April 1, 2004; 18(4): 925 - 940.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Coss, S. B. R. Jacobs, C. E. Bender, and P. L. Mellon
A Novel AP-1 Site Is Critical for Maximal Induction of the Follicle-stimulating Hormone {beta} Gene by Gonadotropin-releasing Hormone
J. Biol. Chem., January 2, 2004; 279(1): 152 - 162.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2003 by The Endocrine Society