| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on May 22, 2003
Accepted on October 30, 2003
1 School of Molecular Biosciences, Center of Reproductive Biology, Washington State University, Pullman, WA 99164
* To whom correspondence should be addressed. E-mail: Griswold{at}mail.wsu.edu,.
FSH and testosterone are required for normal spermatogenesis in mammalian males. These hormones regulate the function of Sertoli cells, which in turn support the differentiation of germ cells in the seminiferous tubules. The molecular targets for these hormones in the testis remain elusive. In this study, we have used hypogonadal (hpg) mice as an in vivo model to examine the actions of testosterone on gene expression in murine testis. This expression pattern was analyzed using Affymetrix Murine GeneChip U74v.2 A, B, C (36,899 transcripts) along with Microarray Suite version 5.0 (MAS 5.0), GeneSpring software and real-time PCR. Hpg mice aged 35-45 days were injected subcutaneously with 25 mg of testosterone proprionate (TP) in 100 mL of sesame oil, and the animals were killed 4,8,12 or 24 h after TP treatments. Untreated hpg mice were used as controls. Gene expression from testes of hpg mice treated with TP was compared with that of testes of untreated hpg mice. At all experimental time points earlier than 24 h, there were more mRNAs with reduced than increased abundance in testes of hpg mice after TP treatment. This study suggests that in murine testis, the primary action of testosterone might be to repress gene expression.
This article has been cited by other articles:
 |
|
 |
|
  M. H. Abel, P. J. Baker, H. M. Charlton, A. Monteiro, G. Verhoeven, K. De Gendt, F. Guillou, and P. J. O'Shaughnessy Spermatogenesis and Sertoli Cell Activity in Mice Lacking Sertoli Cell Receptors for Follicle-Stimulating Hormone and Androgen Endocrinology, July 1, 2008; 149(7): 3279 - 3285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. E Chausiaux, M. H Abel, F. O Baxter, W. T Khaled, P. J.I Ellis, H. M Charlton, and N. A Affara Hypogonadal Mouse, a Model to Study the Effects of the Endogenous Lack of Gonadotropins on Apoptosis Biol Reprod, January 1, 2008; 78(1): 77 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. O'Shaughnessy, M. Abel, H. M. Charlton, B. Hu, H. Johnston, and P. J. Baker Altered Expression of Genes Involved in Regulation of Vitamin A Metabolism, Solute Transportation, and Cytoskeletal Function in the Androgen-Insensitive Tfm Mouse Testis Endocrinology, June 1, 2007; 148(6): 2914 - 2924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Schmidt, J. M. d. Avila, and D. J. McLean Analysis of Gene Expression in Bovine Testis Tissue Prior to Ectopic Testis Tissue Xenografting and During the Grafting Period Biol Reprod, June 1, 2007; 76(6): 1071 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Eacker, J. E. Shima, C. M. Connolly, M. Sharma, R. W. Holdcraft, M. D. Griswold, and R. E. Braun Transcriptional Profiling of Androgen Receptor (AR) Mutants Suggests Instructive and Permissive Roles of AR Signaling in Germ Cell Development Mol. Endocrinol., April 1, 2007; 21(4): 895 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. He, W.-Y. Chan, and M. Dym Microarray technology offers a novel tool for the diagnosis and identification of therapeutic targets for male infertility Reproduction, July 1, 2006; 132(1): 11 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E Nilsson, C. Detzel, and M. K Skinner Platelet-derived growth factor modulates the primordial to primary follicle transition. Reproduction, June 1, 2006; 131(6): 1007 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J Meachem, S. M Ruwanpura, J. Ziolkowski, J. M Ague, M. K Skinner, and K. L Loveland Developmentally distinct in vivo effects of FSH on proliferation and apoptosis during testis maturation J. Endocrinol., September 1, 2005; 186(3): 429 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Anway, M. D. Show, and B. R. Zirkin Protein C Inhibitor Expression by Adult Rat Sertoli Cells: Effects of Testosterone Withdrawal and Replacement J Androl, September 1, 2005; 26(5): 578 - 585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. H Walker and J. Cheng FSH and testosterone signaling in Sertoli cells Reproduction, July 1, 2005; 130(1): 15 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Boekelheide Mechanisms of Toxic Damage to Spermatogenesis J Natl Cancer Inst Monographs, March 1, 2005; 2005(34): 6 - 8. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Kezele, J. M. Ague, E. Nilsson, and M. K. Skinner Alterations in the Ovarian Transcriptome During Primordial Follicle Assembly and Development Biol Reprod, January 1, 2005; 72(1): 241 - 255. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
