help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
This version published online on December 18, 2003
Molecular Endocrinology, doi:10.1210/me.2003-0316
Molecular Endocrinology Vol. 0, No. 2003 200303161-
doi:10.1210/me.2003-0316
Copyright © 2003 by the Endocrine Society.
This Article
Right arrow Author Manuscript (PDF)
Right arrow All Versions of this Article:
18/3/722    most recent
Author Manuscript (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xie, X.
Right arrow Articles by Spencer, D. M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xie, X.
Right arrow Articles by Spencer, D. M
Right arrowPubmed/NCBI databases
*Substance via MeSH

Submitted on August 19, 2003
Accepted on December 12, 2003

The EZC-Prostate Model: Non-invasive prostate imaging in living mice

Xiaoming Xie1, Zheng Luo1, Kevin M Slawin1, and David M Spencer1*

1 Departments of Immunologyand Urology, Baylor College of Medicine, Houston, Texas

* To whom correspondence should be addressed. E-mail: dspencer{at}bcm.tmc.edu.

Recently, progress in the development of prostate-specific promoters and high-resolution imaging techniques has made real-time monitoring of transgenic expression possible, opening a vista of potentially important in vivo models of prostate disease. Herein, we describe a novel prostate reporter model, called the "EZC-Prostate Model" that permits both ex vivo and in vivo imaging of the prostate utilizing a sensitive CCD (charge-coupled device). Firefly luciferase and enhanced green fluorescent protein (EGFP) were targeted to the prostate epithelium using the composite human kallikrein 2 (hK2)-based promoter, hK2-E3/P. In EZC-Prostate mice, the ventral and dorsal/lateral prostate lobes were brilliant green under fluorescence microscopy, with expression localized to the secretory epithelium. In contrast, EGFP was undetectable in the anterior lobes of prostate, seminal vesicles, testes, liver, lung and brain. The kinetics of luciferase activity in intact and castrated living mice monitored with the IVISTM CCD-based imaging system confirmed that firefly luciferase expression was largely prostate restricted, increased with age up to 24 weeks, and was androgen-dependent. Decreases in reporter expression after 24 weeks may reflect well-known, age-related decreases in androgen signaling with age in humans. Ex vivo imaging of micro-dissected animals further confirmed that the luminescence detected in living mice emanated predominately from the prostate with minor signals originating from the testes and cecum. These data demonstrate that the hK2-E3/P promoter directs strong prostate-specific expression in a transgenic mouse model. Multi-genic models, generated by crosses with various hyperplastic and neoplastic prostate disease models could potentially provide powerful new tools in longitudinal monitoring of changes in prostate size, androgen signaling, metastases, or response to novel therapies without sacrificing large cohorts of animals.
Key words: hK2 • prostate • luciferase • EGFP • living imaging




This article has been cited by other articles:


Home page
 Cold Spring Harb Symp Quant BiolHome page
R.U. Lukacs, D.A. Lawson, L. Xin, Y. Zong, I. Garraway, A.S. Goldstein, S. Memarzadeh, and O.N. Witte
Epithelial Stem Cells of the Prostate and Their Role in Cancer Progression
Cold Spring Harb Symp Quant Biol, November 6, 2008; (2008) sqb.2008.73.012v1.
[Abstract] [PDF]

Home page
 Cancer Res.Home page
C.-P. Liao, C. Zhong, G. Saribekyan, J. Bading, R. Park, P. S. Conti, R. Moats, A. Berns, W. Shi, Z. Zhou, et al.
Mouse Models of Prostate Adenocarcinoma with the Capacity to Monitor Spontaneous Carcinogenesis by Bioluminescence or Fluorescence
Cancer Res., August 1, 2007; 67(15): 7525 - 7533.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. R. Seethammagari, X. Xie, N. M. Greenberg, and D. M. Spencer
EZC-Prostate Models Offer High Sensitivity and Specificity for Noninvasive Imaging of Prostate Cancer Progression and Androgen Receptor Action.
Cancer Res., June 15, 2006; 66(12): 6199 - 6209.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. K. Lyons, E. Lim, A. O. Clermont, J. Dusich, L. Zhu, K. D. Campbell, R. J. Coffee, D. S. Grass, J. Hunter, T. Purchio, et al.
Noninvasive Bioluminescence Imaging of Normal and Spontaneously Transformed Prostate Tissue in Mice.
Cancer Res., May 1, 2006; 66(9): 4701 - 4707.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
C-L Hsieh, Z Xie, Z-Y Liu, J E Green, W D Martin, M W Datta, F Yeung, D Pan, and L W K Chung
A luciferase transgenic mouse model: visualization of prostate development and its androgen responsiveness in live animals
J. Mol. Endocrinol., October 1, 2005; 35(2): 293 - 304.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
L. A. Wirtzfeld, G. Wu, M. Bygrave, Y. Yamasaki, H. Sakai, M. Moussa, J. I. Izawa, D. B. Downey, N. M. Greenberg, A. Fenster, et al.
A New Three-Dimensional Ultrasound Microimaging Technology for Preclinical Studies Using a Transgenic Prostate Cancer Mouse Model
Cancer Res., July 15, 2005; 65(14): 6337 - 6345.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2003 by The Endocrine Society