| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on September 10, 2003
Accepted on April 12, 2004
1
Molecular Endocrinology Group, Division of Medicine & MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London, W12 0NN, UK
* To whom correspondence should be addressed. E-mail: graham.williams{at}imperial.ac.uk.
Thyroid hormones are essential for development, growth and metabolism and act via T3 receptors (TR) 
and 
. The THRA and THRB genes have discrete physiological roles but their mRNAs are expressed widely in overlapping patterns. There is poor correlation between TR mRNA and protein, indicating that expression may be regulated by post-transcriptional mechanisms. Differences in the relative levels of expressed TR and proteins have been suggested to modulate tissue T3-responsiveness. We determined the structure of the human THRB gene, cloned seven alternately spliced 5'-untranslated region (5'-UTR) TR1 mRNAs and identified five polyadenylation position elements in the 3'-UTR. At least six TR1 mRNAs between 1.35 and 7.5kb in length were expressed in discrete temporo-spatial patterns in fetal and adult human tissues. The 5'-UTRs contained up to seven upstream short open reading frames, which did not influence the structure of the TR1 protein. In transfection studies, 5'-UTRs exerted cell-specific effects on mRNA expression, but consistently reduced protein expression. Furthermore, each 5'-UTR strongly inhibited translation in vitro. Thus, developmental and tissue-specific expression of human thyroid hormone receptor 1 5'-UTR mRNAs may regulate T3-responsiveness in target tissues by modulating TR protein translation and thereby controlling the ratio of expressed TR and proteins.
NURSA Molecule Pages Link:
This article has been cited by other articles:
 |
|
 |
|
  I. Jones, L. Ng, H. Liu, and D. Forrest An Intron Control Region Differentially Regulates Expression of Thyroid Hormone Receptor {beta}2 in the Cochlea, Pituitary, and Cone Photoreceptors Mol. Endocrinol., May 1, 2007; 21(5): 1108 - 1119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-Y. Liu, R. S. Heymann, F. Moatamed, J. J. Schultz, D. Sobel, and G. A. Brent A Mutant Thyroid Hormone Receptor {alpha} Antagonizes Peroxisome Proliferator-Activated Receptor {alpha} Signaling in Vivo and Impairs Fatty Acid Oxidation Endocrinology, March 1, 2007; 148(3): 1206 - 1217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Khare, A. H. Taylor, J. C. Konje, and S. C. Bell {Delta}9-Tetrahydrocannabinol inhibits cytotrophoblast cell proliferation and modulates gene transcription Mol. Hum. Reprod., May 1, 2006; 12(5): 321 - 333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Conrad, Y. Zhang, A. R. Walker, L. A. Olberding, A. Hanzlick, A. J. Zimmer, R. Morffi, and G. W. Conrad Thyroxine Affects Expression of KSPG-Related Genes, the Carbonic Anhydrase II Gene, and KS Sulfation in the Embryonic Chicken Cornea Invest. Ophthalmol. Vis. Sci., January 1, 2006; 47(1): 120 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Barnard, A. J. Williams, B. Rabier, O. Chassande, J. Samarut, S.-y. Cheng, J. H. D. Bassett, and G. R. Williams Thyroid Hormones Regulate Fibroblast Growth Factor Receptor Signaling during Chondrogenesis Endocrinology, December 1, 2005; 146(12): 5568 - 5580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Hughes and H. J. M. Brady Expression of axin2 Is Regulated by the Alternative 5'-Untranslated Regions of Its mRNA J. Biol. Chem., March 4, 2005; 280(9): 8581 - 8588. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
