help button home button Endocrine Society Molecular Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
This version published online on December 30, 2003
Molecular Endocrinology, doi:10.1210/me.2003-0371
A more recent version of this article appeared on March 1, 2004
This Article
Right arrow Author Manuscript (PDF)
Right arrow All Versions of this Article:
18/3/533    most recent
Author Manuscript (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gerrish, K.
Right arrow Articles by Stein, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gerrish, K.
Right arrow Articles by Stein, R.

Submitted on September 23, 2003
Accepted on December 17, 2003

Conserved Transcriptional Regulatory Domains of the pdx-1 Gene

Kevin Gerrish1, Jennifer C. Van Velkinburgh1, and Roland Stein1*

1 Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 723 Light Hall, Nashville, TN 37215

* To whom correspondence should be addressed. E-mail: roland.stein{at}vanderbilt.edu.

The PDX-1 homeodomain-containing transcription factor affects both pancreatic endocrine cell development and adult islet {beta} cell function. Cell-type-specific expression is controlled by sequences 5'-flanking the pdx-1 gene transcription start site. One principal control region is located roughly between -2800 to -1600 base pairs (bp) and spans three conserved, distinct, and functionally important sub-domains, termed Areas I, II, and III. In this study, we found that an upstream control region in the rat pdx-1 gene located between -6200 to -5670 bp is also present in the mouse, chicken, and human genes. This region is capable of independently directing pancreatic {beta} cell-selective reporter gene expression, and potentiating Area I/II-driven activity. This newly recognized conserved sub-domain has been termed Area IV. The islet-enriched FoxA2, Nkx2.2, and PDX-1 transcription factors have been shown to activate Area IV-driven reporter gene expression as well as bind to this region of the endogenous gene in {beta} cells. Analysis of the histone H3 and H4 acetylation level also indicated that Areas I-IV are within transcriptional active chromatin in {beta} cells. Our data suggests that pdx-1 transcription is also regulated by factors acting upon conserved Area IV sequences.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. M. Vanhoose, S. Samaras, I. Artner, E. Henderson, Y. Hang, and R. Stein
MafA and MafB Regulate Pdx1 Transcription through the Area II Control Region in Pancreatic {beta} Cells
J. Biol. Chem., August 15, 2008; 283(33): 22612 - 22619.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. O. Wiebe, J. D. Kormish, V. T. Roper, Y. Fujitani, N. I. Alston, K. S. Zaret, C. V. E. Wright, R. W. Stein, and M. Gannon
Ptf1a Binds to and Activates Area III, a Highly Conserved Region of the Pdx1 Promoter That Mediates Early Pancreas-Wide Pdx1 Expression
Mol. Cell. Biol., June 1, 2007; 27(11): 4093 - 4104.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Eto, V. Kaur, and M. K. Thomas
Regulation of Pancreas Duodenum Homeobox-1 Expression by Early Growth Response-1
J. Biol. Chem., March 2, 2007; 282(9): 5973 - 5983.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. C. Raum, K. Gerrish, I. Artner, E. Henderson, M. Guo, L. Sussel, J. C. Schisler, C. B. Newgard, and R. Stein
FoxA2, Nkx2.2, and PDX-1 Regulate Islet {beta}-Cell-Specific mafA Expression through Conserved Sequences Located between Base Pairs -8118 and -7750 Upstream from the Transcription Start Site
Mol. Cell. Biol., August 1, 2006; 26(15): 5735 - 5743.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
B. Desvergne, L. Michalik, and W. Wahli
Transcriptional Regulation of Metabolism
Physiol Rev, April 1, 2006; 86(2): 465 - 514.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. Fujitani, S. Fujitani, D. F. Boyer, M. Gannon, Y. Kawaguchi, M. Ray, M. Shiota, R. W. Stein, M. A. Magnuson, and C. V.E. Wright
Targeted deletion of a cis-regulatory region reveals differential gene dosage requirements for Pdx1 in foregut organ differentiation and pancreas formation
Genes & Dev., January 15, 2006; 20(2): 253 - 266.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. C. Van Velkinburgh, S. E. Samaras, K. Gerrish, I. Artner, and R. Stein
Interactions between Areas I and II Direct pdx-1 Expression Specifically to Islet Cell Types of the Mature and Developing Pancreas
J. Biol. Chem., November 18, 2005; 280(46): 38438 - 38444.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. K. Hagman, L. B. Hays, S. D. Parazzoli, and V. Poitout{paragraph}
Palmitate Inhibits Insulin Gene Expression by Altering PDX-1 Nuclear Localization and Reducing MafA Expression in Isolated Rat Islets of Langerhans
J. Biol. Chem., September 16, 2005; 280(37): 32413 - 32418.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2003 by The Endocrine Society