help button home button Endocrine Society Molecular Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
This version published online on June 10, 2004
Molecular Endocrinology, doi:10.1210/me.2004-0103
A more recent version of this article appeared on September 1, 2004
This Article
Right arrow Author Manuscript (PDF)
Right arrow Supplemental Data File
Right arrow All Versions of this Article:
18/9/2255    most recent
Author Manuscript (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow NURSA Molecule Pages Link
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by ARAO, Y.
Right arrow Articles by KAYAMA, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ARAO, Y.
Right arrow Articles by KAYAMA, F.

Submitted on March 10, 2004
Accepted on June 3, 2004

Stability of A+U-Rich Element Binding Factor 1 (AUF1)-Binding Messenger Ribonucleic Acid Correlates with the Subcellular Re-localization of AUF1 in the Rat Uterus upon Estrogen Treatment

YUKITOMO ARAO*, ATSUMI KIKUCHI, MITSUYO KISHIDA, MASAMI YONEKURA, AKIRA INOUE, SHIGEMITSU YASUDA, SEIKI WADA, KAZUHIRO IKEDA, and FUJIO KAYAMA

Department of Environmental Medicine, Center for Community Medicine, Jichi Medical School, Kawachi-gun, Tochigi 329-0498, Japan, School of Agriculture, Ibaraki University, Ami-machi, Ibaraki 300-0393, Japan, Graduate School of Medicine, Osaka City University, Abeno-ku, Osaka 545-8585, Japan and Forth Dept. of Internal medicine, Saitama Medical School, Iruma-gun, Saitama 350-0495, Japan, CREST, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan

* To whom correspondence should be addressed. E-mail: ya{at}jichi.ac.jp.

The nucleo-cytoplasmic shuttling protein AUF1 is one of the RNA binding proteins that specifically bind adenylate-uridylate rich elements (ARE) in mRNA 3'-untranslated regions (UTRs), and acts as a regulator of ARE-mediated mRNA degradation in the cytoplasm. We previously reported that in the female rat uterus, the levels of specific AUF1 isoform mRNAs (p40/p45) were increased by 17{beta}-estradiol (E2) treatment. Therefore, we examined the role of AUF1 in the regulation of E2-mediated mRNA turnover in the rat uterus. We identified ABIN2 and Ier2/pip92 mRNAs as candidate targets of AUF1 in the rat uterus. We found that AUF1 binding elements were present in the 3'-UTR of both mRNAs and that the 3'-UTRs functioned as mRNA turnover regulatory elements. In the OVX rat uterus, the nucleo-cytoplasmic localization of AUF1p40/p37 isoform proteins was regulated by E2. We also found that cytoplasmic AUF1-bound mRNA levels changed coincidentally with the cytoplasmic levels of AUF1p40/p37. Finally, we confirmed that the subcellular localization of AUF1p40 controlled the stability of target mRNAs in vitro, such that cytoplasmically-localized AUF1p40 led to marked mRNA stabilization, while nuclear-localized AUF1p40 stabilized target mRNA only slightly. These results suggested that E2-inducible ARE-containing gene transcripts are regulated at least in part via mRNA stabilization through the nucleo-cytoplasmic re-localization of AUF1.
Key words: AUF1 • nucleo-cytoplasmic shuttling • mRNA stability • uterus • estrogen

NURSA Molecule Pages Link:

Ligands:   17β-Estradiol



This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. H. Ing, D. A. Massuto, and L. A. Jaeger
Estradiol Up-regulates AUF1p45 Binding to Stabilizing Regions within the 3'-Untranslated Region of Estrogen Receptor {alpha} mRNA
J. Biol. Chem., January 18, 2008; 283(3): 1764 - 1772.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
N. H. Ing
Steroid Hormones Regulate Gene Expression Posttranscriptionally by Altering the Stabilities of Messenger RNAs
Biol Reprod, June 1, 2005; 72(6): 1290 - 1296.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2004 by The Endocrine Society