| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on March 16, 2004
Accepted on May 25, 2004
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, United Kingdom.; Department of Obstetrics and Gynaecology, University of Cambridge, The Rosie Hospital, Cambridge, CB2 2SW, United Kingdom.; Microarray Development Group, UK HGMP Resource Centre, Hinxton Hall, Cambridge, CB10 1SB, United Kingdom.; School of Biological Sciences, University of Manchester, M13 9PT, United Kingdom.; Centre for Genome Research, University of Edinburgh, Edinburgh, EH9 3QJ, United Kingdom
* To whom correspondence should be addressed. E-mail: jras100{at}cam.ac.uk.
The endometrium is prepared for implantation by the actions of estradiol and progesterone. In mice the luminal epithelium only becomes fully receptive to the attaching blastocyst in response to the nidatory estrogen surge on day 4 of pregnancy. The cytokine leukemia inhibitory factor (LIF) is rapidly induced by nidatory estrogen and has been shown to be the primary mediator of its action. Implantation fails in the absence of LIF and injection of LIF on day 4 of pregnancy can substitute for the nidatory estrogen. In this study, we sought to identify genes regulated by LIF in the uterine epithelium. We used oligonucleotide microarrays to compare the transcript profiles of paired uterine horns from LIF deficient MF1 mice after intraluminal injection of LIF or PBS on day 4 of pseudopregnancy. Insulin-like growth factor binding protein 3 (IGFBP3) was identified as a gene upregulated by LIF; this was confirmed by RT-PCR. In situ hybridization showed that the primary site of IGFBP3 expression is the luminal epithelium, the known site of LIF action in the uterus. We identified two other genes: amphiregulin and immune response gene-1 (IRG1) whose expression was also upregulated by LIF. IRG1 has recently been shown to be essential for implantation. Expression of all three of these genes in the luminal epithelium is known to be regulated by progesterone. The expression of OSF-2 and L-12/15 Lox, which are also expressed in luminal epithelium under the control of progesterone, were not increased by LIF. This suggests that one of the actions of LIF on luminal epithelium may be to enhance the expression of a subset of progesterone regulated genes.
This article has been cited by other articles:
 |
|
 |
|
  A.A Fouladi-Nashta, L Mohamet, J.K Heath, and S.J Kimber Interleukin 1 Signaling Is Regulated by Leukemia Inhibitory Factor (LIF) and Is Aberrant in Lif-/- Mouse Uterus Biol Reprod, July 1, 2008; 79(1): 142 - 153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Aghajanova, K. Bjuresten, S. Altmae, B.-M. Landgren, and A. Stavreus-Evers HB-EGF but Not Amphiregulin or Their Receptors HER1 and HER4 Is Altered in Endometrium of Women With Unexplained Infertility Reproductive Sciences, May 1, 2008; 15(5): 484 - 492. [Abstract] [PDF]
|
|
|
|
 |
|
 S J Kimber, S F Sneddon, D J Bloor, A M El-Bareg, J A Hawkhead, A D Metcalfe, F D Houghton, H J Leese, A Rutherford, B A Lieberman, et al. Expression of genes involved in early cell fate decisions in human embryos and their regulation by growth factors Reproduction, May 1, 2008; 135(5): 635 - 647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. V. Ingman and R. L. Jones Cytokine knockouts in reproduction: the use of gene ablation to dissect roles of cytokines in reproductive biology Hum. Reprod. Update, March 1, 2008; 14(2): 179 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A Gratao, M. Dahlhoff, F. Sinowatz, E. Wolf, and M. R Schneider Betacellulin Overexpression in the Mouse Ovary Leads to MAPK3/MAPK1 Hyperactivation and Reduces Litter Size by Impairing Fertilization Biol Reprod, January 1, 2008; 78(1): 43 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.A. Campbell, L. O'Hara, R.D. Catalano, A.M. Sharkey, T.C. Freeman, and M. H. Johnson Temporal expression profiling of the uterine luminal epithelium of the pseudo-pregnant mouse suggests receptivity to the fertilized egg is associated with complex transcriptional changes Hum. Reprod., October 1, 2006; 21(10): 2495 - 2513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Dimitriadis, C.A. White, R.L. Jones, and L.A. Salamonsen Cytokines, chemokines and growth factors in endometrium related to implantation Hum. Reprod. Update, November 1, 2005; 11(6): 613 - 630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J Kimber Leukaemia inhibitory factor in implantation and uterine biology Reproduction, August 1, 2005; 130(2): 131 - 145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Catalano, M. H. Johnson, E. A. Campbell, D. S. Charnock-Jones, S. K. Smith, and A. M. Sharkey Inhibition of Stat3 activation in the endometrium prevents implantation: A nonsteroidal approach to contraception PNAS, June 14, 2005; 102(24): 8585 - 8590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Schofield and S. J. Kimber Leukocyte Subpopulations in the Uteri of Leukemia Inhibitory Factor Knockout Mice During Early Pregnancy Biol Reprod, April 1, 2005; 72(4): 872 - 878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. L Lopes, J. A Desmarais, and B. D Murphy Embryonic diapause and its regulation Reproduction, December 1, 2004; 128(6): 669 - 678. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
