| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on June 23, 2004
Accepted on July 26, 2004
Institute of Physiological Biochemistry and Pathobiochemistry, Johannes Gutenberg-University, Medical School, Duesberg Weg 6, 55099 Mainz, Germany
* To whom correspondence should be addressed. E-mail: zechel{at}uni-mainz.de.
The germ cell nuclear factor, GCNF, is essential for normal embryonic development and gametogenesis. To test the prediction that GCNF is additionally required for neuronal differentiation, we used the mouse embryonal carcinoma cell line PCC7-Mz1, which represents an advantageous model to study neuronal cells from the stage of fate choice till the acquirement of functional competence. We generated stable transfectants that express gcnf sense or antisense RNA under the control of a tetracycline-regulated promoter. After retinoic acid- (RA-) induced withdrawal from the cell cycle, sense clones developed a neuron network with changed properties, and the time course of neuron maturation was shortened. Consistent with this data, differentiation of neuronal precursor cells was impaired in antisense cultures. This involved a delay in (i) the down-regulation of nestin, a marker for undifferentiated neuroepithelial cells and stem cells of the central nervous system, and (ii) up-regulation of the somatodendritic protein MAP2 and the synaptic vesicle protein synaptophysin. Neuronal cells in the antisense cultures acquired functional competence, although with a significant delay. Our data propose that the level of GCNF is critical for differentiation and maturation of neuronal precursor cells.
NURSA Molecule Pages Link:
This article has been cited by other articles:
 |
|
 |
|
  M. Hentschke, I. Kurth, U. Borgmeyer, and C. A. Hubner Germ Cell Nuclear Factor Is a Repressor of CRIPTO-1 and CRIPTO-3 J. Biol. Chem., November 3, 2006; 281(44): 33497 - 33504. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-G. Joung, D. Shin, R. H. Seong, and B.-T. Zhang Identification of regulatory modules by co-clustering latent variable models: stem cell differentiation Bioinformatics, August 15, 2006; 22(16): 2005 - 2011. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zechel Requirement of Retinoic Acid Receptor Isotypes {alpha}, {beta}, and {gamma} during the Initial Steps of Neural Differentiation of PCC7 Cells Mol. Endocrinol., June 1, 2005; 19(6): 1629 - 1645. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
