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This version published online on December 21, 2006
Molecular Endocrinology, doi:10.1210/me.2006-0256
Molecular Endocrinology Vol. 0, No. 2006 200602561-
doi:10.1210/me.2006-0256
Copyright © 2006 by the Endocrine Society.
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*Compound via MeSH
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Hazardous Substances DB
*PROGESTERONE

Submitted on June 21, 2006
Accepted on December 1, 2006

The role of Xenopus membrane progesterone receptor {beta} in mediating the effect of progesterone on oocyte maturation

Liat Josefsberg Ben-Yehoshua, Andrea L Lewellyn, Peter Thomas, and James L Maller*

HHMI & Department of Pharmacology, University of Colorado School of Medicine, Denver Colorado;Marine Sciences Institute, University of Texas, Port Aransas, TX

* To whom correspondence should be addressed. E-mail: Jim.Maller{at}uchsc.edu.

Rapid, non-genomic membranal effects of progesterone were demonstrated in amphibian oocytes over 30 years ago. Recently, a distinct family of membrane progestin receptors (mPR) has been cloned in fish and other vertebrate species. In this study we explore the role of mPR in promoting oocyte maturation in Xenopus laevis. RT-PCR analysis indicates that Xenopus oocytes contain transcripts for the mPR{beta} ortholog, similar to what has been reported in zebrafish oocytes, and Western blotting shows that the protein is expressed on the oocyte plasma membrane. Microinjection of mPR{beta}-specific antibodies into oocytes resulted a dramatic inhibition of progesterone-dependent oocyte maturation, whereas microinjection of mRNA encoding Myc-XmPR{beta} resulted in an accelerated rate of progesterone-induced oocyte maturation, concomitant with membranal localization of the protein. Binding studies in mammalian cells expressing XmPR{beta} confirmed specific binding of progesterone by the expressed protein. These results suggest that XmPR{beta} is a physiological progesterone receptor involved in initiating the resumption of meiosis during maturation of Xenopus oocytes.
Key words: oocyte maturation • membrane progesterone receptor • Xenopus

NURSA Molecule Pages Link:

Ligands:   Progesterone



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